Investigation into design of micro flow cytometer data collection system
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Investigation into Design of Micro Flow Cytometer Data Collection System. Sam Weiers. Nastaran Hashemi , Pouya Asrar. Outline. Motivation and objectives Approach, methods Current Status of work/current results Work in progress, planned work and timeline. Motivation/Objectives.

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Investigation into Design of Micro Flow Cytometer Data Collection System

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Investigation into design of micro flow cytometer data collection system

Investigation into Design of Micro Flow Cytometer Data Collection System

Sam Weiers

NastaranHashemi, PouyaAsrar


Outline

Outline

  • Motivation and objectives

  • Approach, methods

  • Current Status of work/current results

  • Work in progress, planned work and timeline


Motivation objectives

Motivation/Objectives

  • Motivation

    • To create a micro flow cytometer that will analyze marine algae in UUVs

  • Objectives

    • Design and construct an electrical system

      • Run multiple electronic components

      • Collect and save data


Approach methods

Approach/Methods

  • First Task: Create a program for data acquisition

  • Tools:

    • LabVIEW 2011

    • NI USB-6351 DAQ Device

    • Excel 2010 (For Graphing)


Approach methods1

Approach/Methods


Approach methods2

Approach/Methods

  • Designed and Created Circuits for:

    • 1 PMT

    • 3 MPPCs


Approach methods3

Approach/Methods

  • Employ the micro channel into the system


Approach methods4

Approach/Methods

  • Employ the micro channel into the system


Current status results

Current Status/Results

  • DAQ program operational and stable

  • All MPPC and PMT circuits working

  • Can only collect data from two sources


Future work planned

Future Work Planned

  • Add light filters to the system

    • Restrict wavelengths entering PMTs and MPPCs

  • Collect data from all four positions

  • Use living organisms instead of beads


Citations

Citations

  • N. Hashemi, J.S. Erickson, J.P. Golden, F.S. Ligler, “Optofluidic Characterization of Marine Algae using a Microflow Cytometer”, Biomicrofluidics, 5, 032009 (2011)

  • PouyaAsrar. Micro Channel Design and Construction.


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