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5’ D -32

In vitro transcription (% wt). (A/TATA)down. TATAdown. G. T. A. C. Adown. Bdown. 5’ D -32. WT. RNA level in vivo (% wt). C. T. A (+1). G. scRNA. G. C. T. TATA. A-block. B-block. SCR1 wt. G. Tn. T. +1. -245. +518. +746. Adown. Tn. +1. Bdown. Tn. +1.

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5’ D -32

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  1. In vitro transcription (% wt) (A/TATA)down TATAdown G T A C Adown Bdown 5’D-32 WT RNA level in vivo (% wt) C T A (+1) G scRNA G C T TATA A-block B-block SCR1 wt G Tn T +1 -245 +518 +746 Adown Tn +1 Bdown Tn +1 1 2 3 4 5 6 Comple- mentation 100 100 ++ 80 5 +/- 5 0 TATAdown Adown Bdown 5’D-32 WT scRNA tRNAIle In vitro In vivo 1 2 3 4 5

  2. Adown Bdown wt T A G C SCR1 wt SCR1 Adown ng TFIIIC 0 50 100 0 50 100 0 50 100 ng TFIIIC +9 0 25 50 100 150 200 300 0 25 50 100 150 200 300 A-block +38 COMPLEX B-block +77 FREE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 Dieci et al., 2002, J. Biol. Chem. 277, 6903

  3. scRNA scRNA scRNA-mini scRNA-mini Galactose-dependent histone H4 expression wt Adown Adown Adown Adown wt wt wt wt glucose shift 1 2 3 4 5 6 7 8 Chromatin disassembly An A-block-mediated, TFIIIC-SCR1 interaction counteracts chromatin repression in vivo Dieci et al., 2002, J. Biol. Chem. 277, 6903

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