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B-lineage. ST-HSC. B220 +. CLP. T-lineage. Sca-1 low cKit low IL-7R  high Flt3 high TdT +. Lin - Sca-1 high cKit + CD34 + Flt3 - Thy1.1 low. Lin - Sca-1 high cKit + CD34 - Flt3 - Thy1.1 low. CD3  + /CD8  +. MPP. Granulocyte lineage. Sca-1 - cKit + IL-7R  - FcR high

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B-lineage

ST-HSC

B220+

CLP

T-lineage

Sca-1low

cKitlow

IL-7Rhigh

Flt3high

TdT+

Lin-

Sca-1high

cKit+

CD34+

Flt3-

Thy1.1low

Lin-

Sca-1high

cKit+

CD34-

Flt3-

Thy1.1low

CD3+/CD8+

MPP

Granulocyte lineage

Sca-1-

cKit+

IL-7R-

FcRhigh

CD34+

Gr-1+

GMP

Macrophage lineage

LT-HSC

CMP

Lin-

Sca-1+

cKithigh

Flt3+

Mac1+

Sca-1-

cKithigh

IL-7R-

FcRlow

CD34+

Erythroid /Megakaryocyte

lineages

Sca-1-

cKit+

IL-7R-

FcRlow

CD34-

MEP

Ter119+

HSCs

HPCs

Committed Lineage

LSK, HSC-enriched

Supplemental Figure 1


c-kit

0.2% MPP

LSK/MPP

A.

Vehicle

Sca-1

Flt3

0.5% CLP

IL-7Ra

CLP

Sca-1

Flt3

0.6% CMP

IL-7Ra

CMP

Sca-1

FcR

CD34

LSK34-/

LSK34+

0.6%

ST-HSC

Thy1.1

0.3%

LT-HSC

Sca-1

Flt3

CD34

Supplemental Figure 2


LSK/MPP

c-kit

0.3% MPP

B.

TCDD

Sca-1

Flt3

0.4% CLP

CLP

IL-7Ra

Sca-1

Flt3

0.2% CMP

CMP

IL-7Ra

FcR

CD34

Sca-1

LSK34-/

LSK34+

0.8%

ST-HSC

Thy1.1

0.2%

LT-HSC

Sca-1

CD34

Flt3

Supplemental Figure 2


Bone marrow cells

Counts (x10-6)

C

A

% Lin- cells

8

LSK

MPP

1

B

% Bone marrow cells

100

Gr-1

24

CMP

Ter-119

20

CLP

2

20

CD3

LSK34-

4

60

B220

2

LSK34+

Days after 5-FU treatment

Days after 5-FU treatment

Mac-1

30

11

16

16

11

9

0

5

3

9

0

5

3

Supplemental Figure 3


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