Sponsored Links
This presentation is the property of its rightful owner.
1 / 102

蛋白质组学 PowerPoint PPT Presentation


  • 139 Views
  • Uploaded on
  • Presentation posted in: General

蛋白质组学. 背 景. 基因数量有限性和基因结构的相对稳定性 VS 生命现象的复杂性和多变性. 从 genomic 到 proteome. 对蛋白质的数量、结构、性质、相互关系和生物学功能进行全面深入的研究已成为生命科学研究的迫切需要和重要任务。. The era of ‘ omics ’ -based science Genomics (基因组学) Post-genomic science (后基因组时代) Functional genomics (功能基因组学) Transcriptomics ( 转录组学) Proteomics (蛋白质组学)

Download Presentation

蛋白质组学

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript



VS

genomicproteome



  • The era of omics-based science

    • Genomics

    • Post-genomic science

      • Functional genomics

        • Transcriptomics

        • Proteomics

        • Metabolomics

      • Structural genomics

        • Aim - 3D structures of all protein folds !




proteomePROTEins + genOMEProteins expressed by a genome

1994WilliamsWilkins



(proteomics)



Proteomics

The study of global changes in protein expression

The systematic study of protein-protein interactions through the isolation of protein complexes


  • The study of proteins expressed by genomes

  • Completion of the sequencing of the 1st draft of human genome indicates there are approximately 250,000 proteins in the human genome

  • Only 2-5% of proteins in human genome have been identified


(functional proteomics)



1996Australia Proteome Analysis FacilityAPAF


NCI1 000

NCIFDA


Celera


1997

1998

19991


1998


2003CHHUPO200392004820058200410


  • 973863








(laser capture microdissectionLCM)


  • two-dimensional electrophoresis2-DE)


  • 10100 kD


2-DE


liquid chromatographyLC

capillary electrophoresisCE





  • 30~100ng2040100

  • PAGE8~10ng

  • PVDF/


  • PAGE

  • 5~15minEDTATris/


  • PVDF

  • PAGE


  • SYPRO Red Orange Ruby

  • SDS-PAGE30~60min2~10ng300nm3

  • SAGE

  • Tris/Edman


  • Tween-20

  • SYPRO Ruby


  • 2-DE


  • :

    MelanieII (BioRad),

    PD Quest (BioRad),

    Phoretix 2D Full, (Amersham Pharmacia Biotech)

    ImageMaster 2d Platinum




m/z

m/z



20

2040

2060

2080

J.J. Thomson

-

-


Sir Joseph John Thomson - 1906Frederick Soddy - 1921Francis William Aston - 1922Hans G. Dehmelt and Wolfgang Paul - 1989Robert F. Curl Jr. & Sir Harold W. Kroto & Richard E. Smalley - 1996C60-C80John Fenn and Koichi Tanaka with Kurt Wuthrich - 2002


Ion source

  • (Electron Ionization EI)

  • (Chemical Ionization , CI )

  • Fast Atomic bombardment, FAB

  • (Electron spray IonizationESI)

  • (Atmospheric pressure chemical Ionization, APCI)

  • Atmospheric pressure photoionization, APPI

  • MALDI-TOF


100,000

10,000

ESI/FAB

MW

APPI

1000

APCI

100

EI/CI

10

Polarity

Very Polar

Nonpolar


  • 80pmol

  • 10,000



(Sequence database search):

(De novo interpretation)


  • , ,

  • , , .

  • , , ,


SWISS-PROT


Mascot Search Engine


Mascot MS/MS Ions Search






1yeast two-hybrid system

  • 1989Field Song


GAL4

  • NNLSGAL1(UASG)

  • CGAL1



  • GAL4 BD AD



  • cDNA


1.

2.

3.


2


1

  • SDSMDLC-ESI-MS/MS


  • bait



2

  • immuno-precipitationIP


p53

  • p53HNE1HNE2

  • SDS-PAGE

  • p53LC-ESI-MS/MS



A.

B.

C.


3

  • TAP Tag


IgG

TEV


EGTA



3. protein chipsprotein array


:

  • -

  • -

  • -


  • Login