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Pre en Probiotica en Darmfunctie

Pre en Probiotica en Darmfunctie. Ger T. Rijkers Depts. Pediatrics & Surgery University Medical Center Utrecht Dept. of Medical Microbiology and Immunology St. Antonius Hospital, Nieuwegein, The Netherlands. Gutflora in numbers (1). Gutflora in numbers (3).

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Pre en Probiotica en Darmfunctie

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  1. Pre en Probiotica en Darmfunctie Ger T. Rijkers Depts. Pediatrics & Surgery University Medical Center Utrecht Dept. of Medical Microbiology and Immunology St. Antonius Hospital, Nieuwegein, The Netherlands

  2. Gutflora in numbers (1)

  3. Gutflora in numbers (3) • 1014 bacteria (x 10> total cells in body) • Approx. 800 species >7000 strains 2.5 x 106 bacterial genes (2.5 x 104 human genes) “It is tempting to suggest that the role of the host is to function as an advanced fermenter, carefully designed to maximize the productivity of the microbiome” Nicholson et al. (Nature reviews microbiology, 2005)

  4. Categories of micro-organisms Gutflora Measles Tetanus

  5. Probiotic bacteria live microbial, food ingredients that are beneficial to health

  6. Just a Selection of Probiotics Bifido bifidum Bifido breve Bifido infantis Bifido lactis Bifido longum Lactobacillus brevis Lactobacillus casei Lactobacillus paracasei Lactobacillus plantarum Lactobacillus helveticus Lactobacillus rhamnosus Lactobacillus salivarius Lactococcus lactis

  7. Probiotics beneficial to health

  8. Probiotics beneficial to health Short sick leave (%) Workers at a packaging plant received daily liquid doses of either a placebo or Lactobacillus reuteri, a bacteria that is cultured in some yogurts, for 80 days. While 23 of the 87 volunteers receiving a placebo reported sick during that period, only 10 out of the 94 volunteers that took the L. reuteri said they were ill. The results of the blind study appear in the journal Environmental Health2. 30 25 20 15 10 5 0 placebo L. reuteri

  9. Probiotics: mechanisms of action Mucosal barrier Pathogen inhibition Immune modulation

  10. Top view VRE VRE + Probiotic Supernatant Side view L.Salivarius W24

  11. PNAS 104 (2007) 7617-7621 Probiotic action of Lactobacillus Lysteria monocytogenes Lactobacillus salivarius L. sal. bacteriocin Abp118

  12. * * Bacterial passage 400 300 200 E.coli passage (fluorescense units) 100 0 Sham AP AP&X AP&Y *= p< 0.05

  13. 70% of your immune system is within your intestine

  14. Dendritische cellen in de darm

  15. Mucosale immuunsysteem in de darm

  16. Mucosale immuunsysteem in de darm

  17. Th1 en Th2 uit balans Autoimmuunziekten Allergische ziekten

  18. Probiotica: klinische effecten Improvement gut microbiota: gastrointestinal function diarrhea Protection against infections Prevention of immune mediated diseases (allergy)

  19. Probiotics: Stabilisation of Gut Flora placebo Probiotics* Amoxicillin Amoxicillin 0 3 7 14 35 63 0 3 7 14 35 63 *Probiotics: B. bifidum, B. lactis, B. longum, E. faecium, L. rhamnosus, L. paracasei L. plantarum (2x), L. acidophilus (2x)

  20. Defaecation classification: Bristol scale

  21. Defaecation classification: Fokke and Sukke scale

  22. Bowel movements

  23. B. infantis 1 x 1010 B. infantis 1 x 108 B. infantis 1 x 106 placebo

  24. Als U wilt diëten, neem wat Bacteriodeten!

  25. Not all probiotics are equal! Lactobacillus acidophilus 109 #55 108 #22 CFU/ml #36 107 106 <105 4 6 0 2 3 5 1 Time (hrs)

  26. Prebiotics Oligosaccharides fermented by bacteria in the colon Stimulates carbohydrate fermentation (SCFA, lactate, acetate, propionate and butyrate) Stimulates growth of Bifidobacteriae and Lactobacilli Reduced protein fermentation (branched chain fatty acids, ammonia, amines, indols, phenols)

  27. Prebiotics stimulates growth of Bifidobacteriae and Lactobacilli Rinne et al. FEMS Immunol Med Microbiol 43 (2005) 59-65

  28. Prebiotics reduce adherence of E.coli to epithelial cell lines Shoaf et al. Infect Immunity 74 (2006) 6920-28.

  29. Prebiotics Moro et al., Arch Dis Child 91 (2006) 814-19

  30. Get sick, get well Hang around a ink well Ring bell, hard to tell If anything is goin' to sell Try hard, get barred Get back, write braille Get jailed, jump bail Join the army, if you fail Look out kid You're gonna get hit But users, cheaters Six-time losers Hang around the theaters Girl by the whirlpool Lookin' for a new fool Don't follow leaders Watch the parkin' meters Subterranean Homesick Blues, Bob Dylan

  31. Ger T. Rijkers Dept. of Pediatrics, Wilhelmina Children’s Hospital Dept. of Surgery, University Medical Center Utrecht Dept. of Medical Microbiology & Immunology Saint Antonius Hospital Nieuwegein The Netherlands ger@rijkers.nl

  32. Surrounded by a world of probiotics

  33. Prebiotics Non-digestible food ingredients that provide beneficial effects to the host by stimulating the growth of selected microbial members of the gastrointestinal tract Mono- and difucosyllactose Lacto-N-tetraose Mono- and di fucosylated derivatives Gefucosyleerd lacto-N-hexaose Lacto-N-octaose Etc

  34. Probiotics (L. reuteri) Anti-gas (Simethicon) 300 300 240 240 180 180 Crying per day (minutes) 120 120 60 60 0 0 0 7 14 21 28 0 7 14 21 28 Treatment (days) Treatment (days)

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