A genomic regulatory network for development
This presentation is the property of its rightful owner.
Sponsored Links
1 / 50

A Genomic Regulatory Network for Development PowerPoint PPT Presentation


  • 72 Views
  • Uploaded on
  • Presentation posted in: General

A Genomic Regulatory Network for Development. Davidson EH, et al. Science 2002 295(5560):1669-1678. Review. מבוא. התפתחות של "תוכנית" הגוף נשלטת בצורה קשיחה ע"י רשת גדולה של גנים רגולטורים. מאמר זה דן ברשת כזאת של התמיינות המסודרם והאנדודרם בעובר קיפוד הים.

Download Presentation

A Genomic Regulatory Network for Development

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


A genomic regulatory network for development

A Genomic Regulatory Network for Development

Davidson EH, et al.

Science 2002 295(5560):1669-1678. Review.


A genomic regulatory network for development

  • "" " .

  • .

  • - , , , - .


A genomic regulatory network for development

""

  • .

  • .

  • ( ) .

  • . , '.


A genomic regulatory network for development

"-"


A genomic regulatory network for development

  • "" .

  • , "" .


A genomic regulatory network for development

  • , .

  • - , "":


A genomic regulatory network for development

1

2

1

  • " cis-regulatory analysis. , -control, , " .

  • . .

'

'


A genomic regulatory network for development

?

  • " " " .

  • , ( ) .


A genomic regulatory network for development

  • -.

  • ' and 2 - " .


A genomic regulatory network for development

  • ( ).

  • .

  • , .


A genomic regulatory network for development

endo16

  • - ( ).

  • " 2 .

  • . " 9 .


A genomic regulatory network for development

  • - , ( )

  • .

  • .


A genomic regulatory network for development

  • ( -50), , -8500.

  • 2 :

  • 1. -

  • .

  • :

  • -. .

  • .


A genomic regulatory network for development

  • 2. . :

  • . , .


A genomic regulatory network for development

?

  • .

  • .

  • -10-12 . "" .


A genomic regulatory network for development

  • - . , . cDNA, BAC.

  • .

  • .

  • - " gene transfer.


A genomic regulatory network for development

  • - endomesoderm .

  • - endomesoderm - mesenchyme ( -micromere), mesoderm .

  • 8 -6 "veg2" -8 "" "veg1".


A genomic regulatory network for development

( ) - skeletogenic lineage.

- the small micromere precursors of adult mesoderm

- aboral ectoderm

- endomesodermal veg2 lineage that later gives rise to endoderm

- endoderm

- mesoderm

- oral ectoderm ( "")


A genomic regulatory network for development

10

15 " -9

24 - - mesenchyme . veg2 , .

55 ( 800 ). ( )


A genomic regulatory network for development

1

veg2

VEG2 -2 :

veg2 4-6.


A genomic regulatory network for development

catenin . catenin Tcf .

catenin


A genomic regulatory network for development

Wnt8

- Wnt8, signal transduction ( ") -catenin/Tcf . .

catenin


A genomic regulatory network for development

  • - "", .

  • " PCR .

  • . <30%,>300% .


A genomic regulatory network for development

GFP

) )

Pmar 1

mRNA fusion

pmar1


A genomic regulatory network for development

GFP

) )

( GFP ...)


A genomic regulatory network for development

Morphilino oligonucleotide.

GFP

) )

mRNA fusion

Pmar 1

pmar1


A genomic regulatory network for development

( ...)


A genomic regulatory network for development

  • " , morpholino-substituted antisense oligonucleotides

  • .

  • ((fusionmRNA mRNA Engrailed( ). .


A genomic regulatory network for development

  • mRNA - , .

  • , -mRNA .


A genomic regulatory network for development

  • , .

  • - .

  • " mRNA .


A genomic regulatory network for development

  • , , , .

  • " Netbuilder , " .


A genomic regulatory network for development

  • , .

  • " - macroarrays.

  • "" " BioArray differential macroarray screens


A genomic regulatory network for development

  • .

  • , 24 .

  • , , .


A genomic regulatory network for development

  • - , " . " .

  • " Interspecific:

  • - S.purpuratus Lytechinus variegatus . . gene transfer .

  • " FamilyRelations.


A genomic regulatory network for development

  • " -3 :

  • Netbuilder, FamilyRelations, BioArray

  • :http://sea-urchin.caltech.edu/software


A genomic regulatory network for development

  • .

  • .

  • .


A genomic regulatory network for development

, :

  • .

  • .

  • .

  • " .


A genomic regulatory network for development

  • .

  • , .


A genomic regulatory network for development

  • " .

  • -, , " .

  • "" .


A genomic regulatory network for development

  • - Bilaterian.

  • .

  • .

  • .


A genomic regulatory network for development

  • - bilaterians " " .

  • .

  • :

  • 1. , .


A genomic regulatory network for development

  • 2. -" " .

  • " , .

  • :

  • ,

  • ,

  • .


A genomic regulatory network for development

  • 30,000 , alternative splicing, '.

  • : .

  • !


A genomic regulatory network for development

....


  • Login