The life and death of bacteria
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The Life and Death of Bacteria. Kelly L. Shipley. Funding and support received from…. Today’s Agenda:. Introduction Safety The Life and Death of Bacteria Basic Practice Techniques Surveying Different Kinds of Media Closing. Introduction:. All living things contain cells

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Today s agenda l.jpg
Today’s Agenda:

  • Introduction

  • Safety

  • The Life and Death of Bacteria

  • Basic Practice Techniques

  • Surveying Different Kinds of Media

  • Closing


Introduction l.jpg
Introduction:

  • All living things contain cells

  • Eukaryotes: more than one cell

  • Prokaryotes: one cell organisms




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What are Bacteria?

  • Bacteria are prokaryotes, meaning they are only ONE celled organisms. They are very small and can be harmful or beneficial.


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Follow along…

  • You have a picture of a bacterial cell at your desk.

  • Follow along with the following diagram, making sure you understand what each structure is and how it works.




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How many bacteria will fit on the head of a pencil, like the one you use every day??

How big are bacteria?



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Bacteria can replicate easily… pencil!!

  • To grow, bacteria divide and divide and divide again.

  • Problem: If you started with only 1 bacteria cell, and it divided 10 times, how many bacteria would you then have??


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Bacteria are everywhere… pencil!!

Don’t panic!!

This is a good thing.

Bacteria are part of our lives, our ecosystem and our habitat!




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As well as those beneficial uses… pencil!!

  • There are many bacteria which humans need every day.

  • They live on us and in us and we NEED them!!!

  • They are called our “NORMAL FLORA”


Normal flora l.jpg
Normal “flora” pencil!!

  • Flora – microorganisms that are

    usually found associated with

    healthy body tissue

  • What does that mean?

    Normal flora are bacteria which our body needs and play a “good” role to help us stay healthy.


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Let’s look at the different areas “flora” live… pencil!!

and then we will test them!!


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Normal flora of the skin pencil!!

  • Skin is considered a “harsh” environment for bacteria, why?

  • Bacteria like the areas of the skin which are moist; underarms, sweat glands etc.

  • Most are not harmful, but Acne…

  • Ie: Staphylococcus, Propionibacterium acnes,


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Normal flora of the oral cavity pencil!!

  • Streptococcus is a normal bacteria in your mouth

  • Other bacteria are also present that turn sugars into acids and cause dental plaque, cavities and decay

  • Brushing our teeth allows the normal flora to grow without build-up of other bacteria!!


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Normal flora of the GI tract pencil!!

  • The GI tract is the stomach, small intestine and large intestine.

  • Several different types of bacteria live in the GI tract and aid in digestion, movement of waste and absorption.

  • Ie: E. coli, Clostridium, Enterococcus faecalis,


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Normal flora of the Urogenital Tract pencil!!

  • Urethra (tube from which you urinate) have normal microorganisms present, but they can become “opportunistic” when there is a change in the environment and causing infection

  • Bladder and reproductive organs should always be sterile


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Normal flora of the Respiratory Tract pencil!!

  • Upper: oral cavity and throat microorganisms live in the areas around mucous membranes

  • Ie: Staph, Strept, and some gram negative

  • Lower: trachea, bronchi and lungs should ALWAYS be sterile, if not – pneumonia


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Lab Information…Pay Attention pencil!!

  • The following is an introduction to some of the media we will be using today…

  • Pay attention!!


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Different Kinds of Media pencil!!

  • We “grow” bacteria on different types of media.

  • “Media” means an environment inside a Petri dish that has certain characteristics

  • We will go over the types of media we are using today


Macconkey agar media l.jpg
MacConkey Agar Media pencil!!

  • MacConkey is specifically designed to help bacteria that live on the skin to grow

  • “Gram Positive” bacteria

  • Grows pink, purple and white colored colonies


Mannitol salt media l.jpg
Mannitol Salt Media pencil!!

  • Mannitol Salt is specifically designed to help “gut”

    organisms grow.

  • “Gram Negative” bacteria

  • Yellow = gut organisms present

    No change = no gut organisms present


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Nutrient Agar Media pencil!!

  • Nutrient media is NOT selective (G+ and G-)

  • This means that most all bacteria LOVE to eat nutrient agar

  • It is the most common media, and we will be using it A LOT!!


Now let s move on to our lab protocol l.jpg
Now, let’s move on to our lab protocol. pencil!!

  • We will be surveying different places where the different “skin” and “gut” bacteria grow

  • Where would be some good places to find either?

  • Where are some places to find them, where they really SHOULDN’T be?


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Anti-Microbial Testing pencil!!

  • Goal: To see how the products we use in our homes affect the different bacteria we know are around.

  • What types of products are out there?


Disinfectants l.jpg
Disinfectants pencil!!

  • Disinfectants are substances that inhibit bacterial growth

  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!


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Disinfectants pencil!!

  • Disinfectants are substances that inhibit bacterial growth

  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!


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Disinfectants pencil!!

  • Disinfectants are substances that inhibit bacterial growth

  • THEY ARE ALSO HARMFUL TO HUMAN TISSUES!!


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Antiseptics pencil!!

  • Antiseptics are chemical substances that inhibit bacterial growth

  • THEY ARE NOT HARMFUL TO HUMAN TISSUES


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Antiseptics pencil!!

  • Antiseptics are chemical substances that inhibit bacterial growth

  • THEY ARE NOT HARMFUL TO HUMAN TISSUES


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Antiseptics pencil!!

  • Antiseptics are chemical substances that inhibit bacterial growth

  • THEY ARE NOT HARMFUL TO HUMAN TISSUES


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What do you think? pencil!!

  • Will antiseptics, which we use on our bodies, be more effective on Gram pos. or Gram neg. bacteria?

  • What about disinfectants?

  • How do each do against the bacteria on our plates?


How do you get your results l.jpg
How do you get your results? pencil!!

  • Call me at 503-838-8209

  • E-mail me at [email protected]

  • Go to our website at www.wou.edu/


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