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Automatic functional transcriptomic annotation Bioinformatic school CIBA

Automatic functional transcriptomic annotation Bioinformatic school CIBA. Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD). Transcriptomic data. Genomic expression changes. Signal. expressed proteins Transcriptomic analysis => functions adaptation.

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Automatic functional transcriptomic annotation Bioinformatic school CIBA

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  1. Automatic functional transcriptomic annotationBioinformatic school CIBA Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD)

  2. Transcriptomic data Genomic expression changes Signal expressed proteins Transcriptomic analysis => functions adaptation

  3. Transcriptomic analysis High-throughput sequencing : => Higher quantity of data. => Lesser cost. Problematic: * How to store data ? * How to analyse data ?

  4. Available tools • ESTtik : • « Expressed Sequence Tag Treatment and Investigation Kit » • Automatic transcriptomic annotation package • Can manage 454 data • Integrate the analysis in a complete database and request web interface. http://esttik.cirad.fr

  5. 454 data Transcripts Random cutting 200 – 800 bases 454 sequencing => ~380 bases Transcript contig assembly Consensus sequence

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