Rodent associated bartonella in saskatchewan
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Rodent Associated Bartonella in Saskatchewan. Claire Jardine ADED Rounds Nov. 26, 2003. Outline. Introduction Objectives Methods Results to date Plans for next year Summary. Bartonella. Gram-negative bacteria Parasitize mammalian RBC Vector-borne. Bartonella. Prior to 1992:

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Rodent Associated Bartonella in Saskatchewan

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Rodent associated bartonella in saskatchewan

Rodent Associated Bartonella in Saskatchewan

Claire Jardine

ADED RoundsNov. 26, 2003


Outline

Outline

  • Introduction

  • Objectives

    • Methods

    • Results to date

    • Plans for next year

  • Summary


Bartonella

Bartonella

  • Gram-negative bacteria

  • Parasitize mammalian RBC

  • Vector-borne


Bartonella1

Bartonella

  • Prior to 1992:

    • Carrion’s disease – B. bacilliformis

    • Trench fever – B. quintana

      “medical curiosities”

      “bacteria from the past”


Bartonella2

After 1992:

Urban trench fever

Cat scratch disease

Bacillary angiomatosis

Parinaud’s oculoglandular syndrome

Peliosis hepatis

Uveitis

Encephalitis

Endocarditis

Febrile illness

Neuroretinitis

Myocarditis

Bartonella


Bartonella3

Bartonella


Objectives

Objectives

  • Determine if Bartonella occurs in selected small mammals in central Saskatchewan

  • Determine which isolates of Bartonella are infecting small mammals in central Saskatchewan


Study areas

Study Areas

  • Ten sites within 50 km radius of Saskatoon


Culture

Culture


Rodent associated bartonella in saskatchewan

500 bp

Samples 2-9

N

P

PCR


Phylogeny

Phylogeny


Rodent associated bartonella in saskatchewan

Plan

  • Complete PCR, sequencing work

  • Develop phylogeny for all isolates


Objective

Objective

  • Investigate the ecology of Bartonella infections in RGS


Methods

Methods

  • Each month at 5 sites

  • Trap

  • Collect blood

  • Mark

  • Release


Results

Results


Plans

Plans

  • Demographics

  • Follow individual animals

  • Relate Bartonella prevalence to flea prevalence and load

  • Determine the effect of experimentally reducing flea loads on Bartonella


Summary

Summary

  • Bartonella occurs at high prevalence levels in small mammals in Saskatchewan

  • New strains associated with Richardson’s ground squirrels

  • Plan

    • Ecology

    • Fleas


Rodent associated bartonella in saskatchewan

“The overall host-species diversity of Bartonella attests to the ability of members of this genus to adapt to a wide range of vertebrate hosts. This observation suggests that various members of the genus, not currently associated with human disease, are likely to continue to evolve to be sources of zoonotic and perhaps emergent human disease.”

Karem et al. 2000


Acknowledgements

Funding/Support

MRC, WHF, MSFHR

CDC, Fort Collins

National Microbiology Lab, Winnipeg

CCWHC/WCVM

CWS

Committee

Dr. Leighton

Dr. Wobeser

Dr. Chirino

Dr. Artsob

Dr. Bollinger

Dr. Jackson

Acknowledgements

Special Thanks:

Dr. Kosoy from the CDC, Dr. Appleyard and the PCR lab, Dr. Chirino’s lab, and D. McColl from Health Canada


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