細胞穿透胜肽之分子運送 Molecular Delivery of Cell-Penetrating Peptides. 李漢榮 國立東華大學 自然資源與環境學系. Speaker: Dr. Han-Jung Lee Department of Natural Resources and Environmental Studies National Dong Hwa University. b -Gal. Introduction. PTD. Extracellular. Intracellular. Plasma Membrane.
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細胞穿透胜肽之分子運送Molecular Delivery of Cell-Penetrating Peptides
Speaker: Dr. Han-Jung Lee
Department of Natural Resources and Environmental Studies
National Dong Hwa University
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Transactivator (Tat) protein of the human immunodeficiency virus type 1 (HIV-1) was able to enter into cells and activate HIV-LTR.
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Basic amino acid-rich domain
CPPs, also called protein transduction domains (PTDs), are a group of short, highly basic peptides which can penetrate into cells either alone or associated with cargoes.
Chang et al., Plant Cell Physiol. 46: 482-488, 2005
Covalent protein transduction into onion root tip cells
(tomato root cells)
(onion epidermal cells)
Covalent protein transduction in plant cells
covalent protein transduction (CPT)
noncovalent protein transduction (NPT)
Noncovalent protein transduction in onion root cells
Chang et al., New Phytol. 174: 46-56, 2007
CPPs are able to deliver proteins into cells in a covalent protein transduction (CPT) and noncovalent protein transduction (NPT).
Chen et al., FEBS Lett. 581: 1891-1897, 2007
Wang et al., J. Biochem. Biophy. Methods 70: 579-586, 2007
CPPs can deliver proteins, DNAs and RNAs into cells in a noncovalent manner.
Hu et al., Peptides 30: 1669-1678, 2009
CPPs are able to deliver proteins into cells in a covalent protein transduction (CPT), noncovalent protein transduction (NPT) and covalent and noncovalent protein transductions (CNPT).
Liu et al., Biomaterials 32: 3520-3537, 2011
We demonstrate that CPPs can effectively deliver proteins, DNAs, RNAs and nanoparticles into live cells.
However, the mechanism of protein transduction is unknown yet.