Involvement of the Mitochondrial Calcium Uniporter in Cardioprotection by Ischemic Preconditioning. Background.
Ischemic preconditioning (IPC, which was originally identified by Murry et al.) provides powerful protection to the ischemic myocardium. Although a variety of intracellular signaling pathways have been implicated in this protection, the precise mechanisms remain elusive.
An important phenomenon: Cardioprotection by Ischemic Preconditioning
The close relationship among IPC , the mitochondrial calcium concentration and the improved contractile function of heart.
A guess Cardioprotection by Ischemic Preconditioning
There might be certain relationship between ischemic preconditioning and calcium transport system of mitochondrial.
Mitochondrial permeability transition pore (MPTP) and ischemic preconditioning
Mitochondrial calcium uniporter ischemic preconditioningplays important role in mediating the enter of calcium into the mitochondrial.
Questions: ischemic preconditioning
1.Whether activity of mitochondrial calcium uniporter is involved in ischemic preconditioning?
2.And how the relationship between the mitochondrial calcium uniporter and the mitochondrial permeability transition pore?
Part one: Isolated heart experiment ischemic preconditioning
Perfusion protocols used for hemodynamic measurements in isolated rat hearts.
Infarct size was determined by the 2,3,5-triphenyltetrazolium chloride (TTC) staining method. Infarct size was expressed as percentage of risk zone.
Results isolated rat hearts
Contractile functional change of isolated rat hearts:
Effects of ischemia/reperfusion (I/R), ruthenium red (RR), Ru360, spermine (Sper), cyclosporin A (CsA) and ischemic preconditioning (IPC) on LVDP (A), dP/dtmax (B and C) and LVEDP (D) at 30 min of reperfusion following 30 min of ischemia in isolated rat hearts.
Effects of ischemia/reperfusion (I/R), ruthenium red (RR, 5 isolated rat heartsM), Ru360 (10 M), spermine (Sper, 20 M), cyclosporin A (CsA, 0.2 M) and ischemic preconditioning (IPC) on infarct size in isolated rat hearts after 120 min of reperfusion following 30 min of ischemia
Effects of ischemia/reperfusion (I/R), ruthenium red (RR, 5 isolated rat heartsM), Ru360 (10 M), spermine (Sper, 20 M), cyclosporin A (CsA, 0.2 M), ischemic preconditioning (IPC) and IPC+Sper ( 20 M) on lactate dehydrogenase (LDH) release during reperfusion in isolated rat hearts.
The effects of ruthenium red (RR), Ru360, spermine (Sper), cyclosporin A plus spermine (CsA + Sper), ischemic preconditioning (IPC) and IPC plus spermine treatment, on pore opening.