Mold track n trace
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Mold Track `n Trace. Mold’ly going where no man has gone before. iGEM 2011 project proposal – Wageningen UR 2011. A. nidulans. Following a signal (eGFP) through a hypha Diffusion of signal carrier through septa. Project - Hyphae. Project - Hyphae. Initial stimulation. Project - Hyphae.

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Mold Track `n Trace

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Mold track n trace

Mold Track `n Trace

Mold’ly going where no man has gone before

iGEM 2011 project proposal – Wageningen UR 2011


A nidulans

A. nidulans

Following a signal (eGFP) through a hypha

Diffusion of signal carrier through septa


Project hyphae

Project - Hyphae


Project hyphae1

Project - Hyphae

Initial stimulation


Project hyphae2

Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup


Project hyphae3

Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup

Carrier diffuses

Process start again in cell N+1


Project hyphae4

Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup

Carrier diffuses

Process start again in cell N+1

etc.


Project colony

Project - Colony


Project colony1

Project - Colony


Project colony2

Project - Colony


Project colony3

Project - Colony


Project colony4

Project - Colony


Project colony5

Project - Colony


Proposed mechanisms

Proposed mechanisms

xpna

eGFP-Svac

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

xpna

XlnR*


Proposed mechanisms1

Proposed mechanisms

UASgal4

eGFP-Svac

GAL4

UASgal4

GAL4


Gal4 regulation

GAL4 regulation

3c

3c

3c

3c

80

80

80

80

80

80

4

4

4

4

4

4

10bp

10bp


Combination

Combination

3c

3c

3c

XpnA

GAL3c

80

80

4

4

XpnA

XlnR*

eGFP-Svac


S w o t

S.W.O.T


S c o t

S.C.O.T

New model organism, A. nidulans

Visual signal (GFP)

System can be subdivided (modular)

Proposed RR have been used before

Some are even BioBricked

In house knowledge about A. nidulans


S c o t1

S.C.O.T

Keeping noise from ruining system

Tuning the system

Lots of constructs need to be made


S c o t2

S.C.O.T

Tuning and noise reduction using the GAL system

Opportunity to gain fundamental knowledge about A. nidulans hyphae

Opportunity to broaden the scope of synthetic biology BioBricks


S c o t3

S.C.O.T

Long period from transformation until measuring of results, 1 Month.

Unknown synthetic territory

Eukaryote chassis


Questions

Questions

?


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