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Mold Track `n Trace. Mold’ly going where no man has gone before. iGEM 2011 project proposal – Wageningen UR 2011. A. nidulans. Following a signal (eGFP) through a hypha Diffusion of signal carrier through septa. Project - Hyphae. Project - Hyphae. Initial stimulation. Project - Hyphae.

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Mold Track `n Trace

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Mold Track `n Trace

Mold’ly going where no man has gone before

iGEM 2011 project proposal – Wageningen UR 2011


A. nidulans

Following a signal (eGFP) through a hypha

Diffusion of signal carrier through septa


Project - Hyphae


Project - Hyphae

Initial stimulation


Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup


Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup

Carrier diffuses

Process start again in cell N+1


Project - Hyphae

Initial stimulation

First signal produced

Carrier buildup

Carrier diffuses

Process start again in cell N+1

etc.


Project - Colony


Project - Colony


Project - Colony


Project - Colony


Project - Colony


Project - Colony


Proposed mechanisms

xpna

eGFP-Svac

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

XlnR*

xpna

XlnR*


Proposed mechanisms

UASgal4

eGFP-Svac

GAL4

UASgal4

GAL4


GAL4 regulation

3c

3c

3c

3c

80

80

80

80

80

80

4

4

4

4

4

4

10bp

10bp


Combination

3c

3c

3c

XpnA

GAL3c

80

80

4

4

XpnA

XlnR*

eGFP-Svac


S.W.O.T


S.C.O.T

New model organism, A. nidulans

Visual signal (GFP)

System can be subdivided (modular)

Proposed RR have been used before

Some are even BioBricked

In house knowledge about A. nidulans


S.C.O.T

Keeping noise from ruining system

Tuning the system

Lots of constructs need to be made


S.C.O.T

Tuning and noise reduction using the GAL system

Opportunity to gain fundamental knowledge about A. nidulans hyphae

Opportunity to broaden the scope of synthetic biology BioBricks


S.C.O.T

Long period from transformation until measuring of results, 1 Month.

Unknown synthetic territory

Eukaryote chassis


Questions

?


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