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Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells

Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells. http://www.youtube.com/watch?v=_-9pROnSD-A. Introduction. Small noncoding RNAs have important regulatory functions in different cell pathways. post-transcriptional regulation

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Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells

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  1. Distinct MicroRNA Subcellular Size and Expression Patterns in Human Cancer Cells http://www.youtube.com/watch?v=_-9pROnSD-A

  2. Introduction • Small noncoding RNAs have important regulatory functions in different cell pathways. • post-transcriptional regulation • Recent evidence suggests miRNA and siRNA activity in the nucleus. • distinct genome-wide sub-cellular localization distribution profiles of small noncoding RNAs in human breast cancer cells.

  3. Methods • separated breast cancer cell nuclei from cytoplasm, and identified small RNA sequences • a high-throughput sequencing platform. • microarray analysis to examine the miRNA expression levels in nucleus • Logistic regression and SVM were used for further analysis

  4. Results • not only locate to the cytoplasm but also appeare in the nucleus. • microarray analyses indicated that the miRNA nuclear-cytoplasmic-ratio is a significant characteristic of different cancer cell lines

  5. Conclusions • the sub-cellular distribution is important for miRNA function • characterization of the small RNAs sub-cellular localizome may contribute to cancer research and diagnosis.

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