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Morphology Development. Viral suspension C monomer (t) . Spacer 1-200 m m. Encapsulant. Fixation UV-crosslink & encapsulation . 1 cm. Vapor Diffusion Drop Method . glass crystallization dish. UV monomer. Viral suspension (<5% monomer). Concentrate II Vapor Diffusion

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Slide1 l.jpg

Morphology Development

Viral suspension

Cmonomer(t) 

Spacer

1-200 mm

Encapsulant

Fixation

UV-crosslink &

encapsulation

1 cm

Vapor Diffusion Drop Method

glass

crystallization

dish

UV monomer

Viral suspension

(<5% monomer)

Concentrate II

Vapor Diffusion

Drop Method

(24 hr)

Pelletize

18K RPM

25-30 min

Concentrate I

Decant

K521 (UV-resin)

Re-suspend

Kramer


Slide2 l.jpg

M0

Chimeric Virus and Virus-Like

Particle Materials

Viral Scaffold

Monodisperse

Heterogeneous, site specific surface chemistry

Shape & Size

Cowpea Chlorotic

Mottle Virus (CCMV)

T. Douglas et al.

Adv. Mat. (2002) 14: 415-418.

M. Stone


Slide3 l.jpg

1

7:3 (Ag:Au)

(resonance condition)

Abs.

Nanoshells have a strong EM near-field at the plasmon resonance frequency

0

8.00

400

600

800

1000

7.00

Wavelength (nm)

6.00

5.00

Extinction (arb. units)

Abs.

4.00

3.00

2.00

Virus core

400

500

600

700

800

900

1000

1.00

Silica core

Wavelength (nm)

0.00

400

500

600

700

800

900

Wavelength (nm)

Radloff, Halas et al

Optical Properties of Gold Colloid and Coated Dielectric Sphere

The cup is green, but looks red in Transmission.

Absorbance spectrum of gold colloid:

G. Mie, Ann. Physik 25, (1908) 377

Lycurgus Cup

4th Century A.D., Roman

(www.thebritishmuseum.ac.uk/science)


Slide4 l.jpg

1

7:3 (Ag:Au)

(resonance condition)

Abs.

0

400

600

800

1000

Wavelength (nm)

Abs.

Virus core

400

500

600

700

800

900

1000

Silica core

Wavelength (nm)

Radloff, Halas et al

Optical Properties of Gold Colloid and Coated Dielectric Sphere

The cup is green, but looks red in Transmission.

Absorbance spectrum of gold colloid:

G. Mie, Ann. Physik 25, (1908) 377

Lycurgus Cup

4th Century A.D., Roman

(www.thebritishmuseum.ac.uk/science)


Slide5 l.jpg

Improved Seed Coverage on Virion Cores

Increasing the ionic strength of the gold nanoparticle solution decreases the repulsive forces between particles allowing for increased surface coverage.

gold decorated IV nanoparticles


Slide6 l.jpg

Gold Nanoshell Growth on Virion Cores

The evolution of the nanoshell plasmon resonance can be followed spectroscopically

dipole

resonance

quadrupole

resonance


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BioHarvesting Natural Forms

For Photonics

Introduction: Photonic Band Gap Materials

Bio-Templating

Scaffolds and Top-Down Replication

Bio-Colloids

Self, Forced & Directed Assembly

Summary


Slide8 l.jpg

Summary

Demonstrated feasibilityandpotential of Bio-Harvesting non-optical natural forms for Photoincs

Bio-Templating:

Proof-of-concept

High index refraction P-Surface for VLWIR exo-atmospheric missile defense apps.

Detail optical characterization

Spatial limits of size-reduction scheme

Exo- and indo-skeletal biotemplates for MIR & VLWIR applications

Bio-Colloids:

Proof-of-concept

Large viral capacity

Numerous ‘assembly’ approaches

Patterning

Single crystal growth

Surface conjugation

High-index replication for nonlinear optical applications (sensor platform, radar, limiting, laser emission)



The form structure factor l.jpg

R2

R1

R3

RHS

The Form & Structure Factor

  • Form factor, P(q):

  • To describe the shape of the IV, we can use a 3-layer core shell model with varying electron densities, the form factor expression is a modified expression (incorporating gradual changes in electron densities) developed by Roche et al.1:

  • Structure factor, S(q):

  • The Percus-Yevick Hard-Sphere Model is an approximation of the pair-correlation function2:

1: E. J. Roche, R. S. Stein, T. P. Russell, W. J. MacKnight (1980) Journal of Polymer Science: Part B Polymer Physics18, 1497-1512.

2: D. J. Kinning, E. L. Thomas (1984) Macromolecules17, 1712-1718.


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Morphology of Pelletized WIV: Local Form of IV

R2

R1

R3

RHS

adapted from N.G. Wrigley 1969

(J. Gen. Virol. 5:123-134)

cryoTEM, negative starining

135 nm

  • Electron micrograph of IV show a electron dense region of the core and the shell

Terje Dokland

  • Representative Model consists of:

  • RHS hard sphere radius due to volume exclusion

  • R1 outer radius of the IV

  • R2- R3 thin region with low electron density

  • R3 core radius of IV

Chan


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Dehydrated, cross-linked WIV

Ultra Small Angle X-ray Scattering

R2

R1

R3

RHS

For the Fitting:

RHS = 790 Å

R3 = 590 Å

R2 = 680 Å

R1 = 710 Å

fHS = 31%

S(q): P-Y

P(q): Hard Sphere

S(q): P-Y

P(q): Core-Shell

S(q): P-Y

P(q): Concentric Shells

Chan


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