1 making density gradients
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1- Making Density Gradients. Pre-formed discontinuous gradients Pre-formed continuous gradients Self-generated gradients. 2- Density Gradient Harvesting. C. A. B. Discontinuous gradient by overlayering. A. B. C. D. Discontinuous gradient by underlayering. density. top. bottom.

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1 making density gradients
1- Making Density Gradients
  • Pre-formed discontinuous gradients
  • Pre-formed continuous gradients
  • Self-generated gradients

2- Density Gradient Harvesting

gradient master profiles from 10 and 40 iodixanol at 80 and 20 rpm effect of time

Density (g/ml)

2 min

1.13

2.5 min

3.0 min

1.11

1.09

1.07

1

3

5

7

9

Fraction Number

Gradient Master profiles from 10% and 40% iodixanol at 80° and 20 rpm: effect of time
self generated gradients

time

g

Self-generated gradients
  • At 360,000gav iodixanol molecules will start to sediment
iodixanol self generated gradient requirements
Iodixanol self-generated gradient requirements
  • Rotors with a short sedimentation path length ~ 17 mm
  • RCF of 180,000-350,000gav
effect of time on iodixanol self generated gradient in the beckman tln100 at 365 000g

Density (g/ml)

15% iodixanol

1.30

1 h

3 h

1.25

20% iodixanol

1.20

1 h

3 h

1.15

1.10

1.05

1.00

1

3

5

7

9

11

13

15

Fraction Number

Effect of time on iodixanol self-generated gradient in the Beckman TLN100 at 365,000g
effect of rcf and iodixanol in beckman vti65 1 3 h

1.3

15% (170,000g)

30% (170,000g)

15% (353,000g)

1.2

30% (353,000g)

Density (g/ml)

1.1

1.0

1

3

5

7

9

11

Fraction Number

Effect of RCF and [iodixanol] in Beckman VTi65.1 (3 h)
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