Microbiological remediation of chlorinated hydrocarbons in contaminated soils. Széchenyi plan NKFP 3/007/2001. Identifying the main goal or goals of the project. The aim of the project is to work out bioremediation technology for cleaning soils polluted by chlorinated hydrocarbons.
The aim of the project is to work out bioremediation technology for cleaning soils polluted by chlorinated hydrocarbons.
Chlorinated hydrocarbons are the most frequent persistent pollutants in Hungary. For example huge amount of waste containing chlorophenols and chlorinated pesticide residues are deposit in Balatonfűzfő. After transporting the barrels containing polychlorinated benzene from Garé the soil remaining is polluted with these compounds. Furthermore, chlorobenzene polluted soils can be found in Hidas region in an industrial area of Budapest Chemical Company. Even today large quantity of chlorinated intermediers and solvents are used in chemical industry.
In order to fit the concentration of chlorinated hydrocarbons and biodegradation technology in the firs part of the project we estimate the pollution of chlorinated hydrocarbon is known legal and illegal deposition sites.
Prallel to this work we work out modern analytical methods (GC, GC-MS, HPLC, HPLC-MS, HPLC-MS-MS) for monitoring biodegradation process and for isolation and structural elucidation of metabolites formed during the procedure.
Distribution of the pollutants (degradation products of biotic and abiotic process) is determined by the type of the soil. Experimental work in determination of sorption capacity of different soils will give data about it.
Research in biodegradation of chlorinated hydrocarbons is the base of the selection of strains or composite of strains for field experiments.
Last but not least a closed mobile plastic redemption house can be built.
Summary of the aims of the project is the following:
- Determination of condition of pollution
- Developing analytical methods
-Selection of bacteria and filamentous fungi strains for degradation of chlorinated hydrocarbons
- Soil sorption experiments and working out distribution model
- Application of biodegradation in the polluted field small and large scale up to 100 tones
Building a closed and mobile house for bioremediation of polluted soils.
TUB Department of Agricultural Chemistry and Technology
bacteria: TUB B-645 TUB B-671
microspores::TUB F-1224 TUB F-1234, TUB F-1238 – TUB F-1239, TUB F-1260 – TUB F-1261
The strains were lyophilized and placed in the microbiological collection of the TUB.
TUB Department of General and Analytical Chemistry
Development of the proper analytical methods (GC, GC-MS, HPLC) for the following of the biodegradation are ready. The selection of the strains happened on the base of these analytical results.
University of Debrecen, Medical and Health Science CenterDepartment of Human Genetics
During the research was isolated 18 strains. Morphological investigation was done with 13 strain and was developed methods for the storing of the strains. In the investigated period (5 months) with one of the storing method was possible the storing without decreasing of the population.
Biological experiments for degradation of chlorobenzenes: