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Microbiology Overview. KWWOA April 09, 2014 Department for Environmental Protection Energy & Environment Cabinet. Topics. Topics. Proficiency Tests Chain of Custody Methods Membrane Filtration. Proficiency Tests. Proficiency Tests. Drinking Water: WS Study SW sample for MPN

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microbiology overview

Microbiology Overview

KWWOA

April 09, 2014

Department for Environmental ProtectionEnergy & Environment Cabinet

topics1
Topics
  • Proficiency Tests
  • Chain of Custody
  • Methods
  • Membrane Filtration
proficiency tests1
Proficiency Tests
  • Drinking Water: WS Study

SW sample for MPN

  • Wastewater: DMR-QA

WP Study

  • KY Approved PT Providers List
proficiency tests5
Proficiency Tests

Revised reports will not be accepted.

Photo: peoplesdaily.com

chain of custody1
Chain of Custody
  • Client name & address
  • Sampler name
  • Sample location
  • Collection date & time
  • Sample type (grab/composite)
  • Matrix (DW/WW)
  • Field sample ID
  • Laboratory sample ID
  • Container type, & size/volume
  • Number of containers
  • Preservative (ice, etc.)
  • Field data (pH, TRC, etc.)
  • Requested analysis
  • Reason for rejection or
  • special remarks
  • Signature, date & time for
  • each transaction
  • (relinquishing & receiving)
  • Sample temperature upon arrival at lab
colilert 18 p a
Colilert-18 P/A

Colilert-18pre-warming

membrane filtration1
Membrane Filtration

Dilutions:

Select appropriate volumes

Fecal Coliform or E. coli colonies: 20-60*

Total colonies: 200

*Use range as specified in particular method.

Photo: www.pall.com

membrane filtration2
Membrane Filtration

Blanks:

  • Beginning of series
  • End of series
  • RECORD results of blanks
membrane filtration3
Membrane Filtration

Calculating Results:

  • Use dilution that is in range to determine result.
  • If multipleplateswithin range, average those plate counts.
  • If zero colonies on all plates, calculate as if 1 colony on filter from largest volume processed. Include remark “<“ in comments.

Photos: www.avocetlab.com

membrane filtration4
Membrane Filtration

Calculating Results:

  • If all plates are above range, calculate using filter from smallest volume processed. Include remark “Estimated“ in comments.
  • WW TNTC =

60,000

contact information
Contact Information

Tiffany Nolan

Kentucky Department for Environmental Protection

Division of Water

200 Fair Oaks Lane; 4th Floor

Frankfort, KY 40601

Phone: (502) 564-3410

Email: [email protected]

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