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Figure 1. shGld2+ Gld2-HA. a. b. Mock. shGld2. Gld2-HA. Gld2-HA. Gld2. Tubulin. Tubulin. c. d. shGld2. shGld2. Mock. shGld2. Mock. p53. A 200. Actin. A 0. e. shGld2. f. Mock. shGld2. 8. 7. Mock. 6. 5. Oxygen Consumption (AU). shCPEB. 4. 10. Mock. 8. 3.
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Figure 1. shGld2+ Gld2-HA a b Mock shGld2 Gld2-HA Gld2-HA Gld2 Tubulin Tubulin c d shGld2 shGld2 Mock shGld2 Mock p53 A200 Actin A0 e shGld2 f Mock shGld2 8 7 Mock 6 5 Oxygen Consumption (AU) shCPEB 4 10 Mock 8 3 shGld2 Population Doublings 6 2 4 1 2 0 0 0 75 15 30 45 60 90 0 3 6 9 Time (min) Days
Figure 2. a b TK-Rluc CPEB-Full-3’-UTR 500 CPEB-Full-5’-Half TK-Rluc ΔB 400 TK-Rluc Relative % to mock (normalized to actin) ΔC TK-Rluc 300 ΔD TK-Rluc 200 ΔE TK-Rluc 100 Fluc control TK-Fluc 0 CPEB Intron CPEB Exon d c 400 CPEB-Full-3’-UTR CPEB-Full-5’-Half 200 300 150 Relative % (Rluc/Fluc) Relative % (Rluc/Fluc) 200 100 100 50 0 0 Full Mock shGld2 ΔB ΔC ΔD ΔE
Figure 3. a d TK-Rluc CPEB-Full-3’-UTR 12 Relative mir122 exp. (normalized to actin) 10 TK-Rluc Δ122-1 8 TK-Rluc Δ122-2 6 TK-Rluc Both 4 2 Fluc control TK-Fluc 0 shGld2 WT Anta-122 Anta-Scr 250 Mock e 200 Relative % (Rluc/Fluc) 150 35S-p53 100 50 Both Δ122-1 Δ122-2 0 WCE CPEB-Full f b 200 150 % p53 protein 100 50 0 Mock Anta-122 Anta-Scr c shTETR shCPEB g 400 Anta-Scr Anta-Scr Anta-122 Anta-122 Mock Mock 300 Relative % (Rluc/Fluc) 35S-p53 200 100 0 Mock WCE Anta-122 Anta-Scr
Figure 4. shMPAP shGld4 Mock d a shMPAP Mock shGld4 A200 p53 A0 Actin e Intron Exon b 200 shTETR shCPEB p53 expression (relative to actin) 150 100 50 IP Flag-Gld4 IP Flag-Gld4 20% input 20% input 0 IgG IgG Mock Mock shMPAP shMPAP shGld4 shGld4 p53 GAPDH f Mono-adenylated miR122 Gld2 miR122-RISC A A A 5% input IgG IP CPEB c CPEB up CPEB down A A CPEB CPEB Gld4-HA p53 down p53 up Gld4 Mcl1-HA CPEB p53 p53 AAAAAAAAAAA A
