Sterilization methods
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Sterilization Methods. Microbiology Biology II Mrs. Hieneman. Terminology. Sterilization – complete elimination of microbial viability Sterilant – chemical agent that sterilizes Disinfection – killing, inhibition or removal of disease-causing microbes.

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Sterilization Methods

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Sterilization methods

Sterilization Methods

Microbiology

Biology II

Mrs. Hieneman


Terminology

Terminology

  • Sterilization – complete elimination of microbial viability

  • Sterilant – chemical agent that sterilizes

  • Disinfection – killing, inhibition or removal of disease-causing microbes.

  • Disinfectant – chemical agent that disinfects

  • Antiseptic – chemical agents applied to tissues which prevent sepsis

  • Sanitation – establishment of environmental conditions favorable to health.


Terminology1

Terminology

  • -cide: to kill

    • Germacide

    • Bactericide

    • Fungicide

  • -static: to inhibit growth

    • Bacteriostatic

    • Fungistatic


Oxygen requirements for microbes

Oxygen Requirements for Microbes

  • Obligate aerobes – grow only in the presence of oxygen.

  • Strict anaerobes – grow only in the absence of oxygen.

  • Facultative anaerobe – can grow in presence (best) or absence of oxygen.

  • Microaerophilic – require small amount of oxygen (about 5%), inhibited by normal (oxygen) of atmosphere.

  • Aerotolerant – oxygen?? Who cares!!!!


Oxygen requirements for microbes1

Oxygen Requirements For Microbes


Chemical agents

Chemical Agents

  • Work by disrupting cellular membranes, oxidizing macromolecules, denaturing proteins.

  • Include disinfectants and antiseptics

  • Classified base upon effectiveness:

    • High Level – effective against all life

    • Intermediate Level – defined as tuberculocidal, as well as more resistant viruses (hepatitis C virus)

    • Low Level – kill vegetative cells of bacteria and fungi and enveloped viruses.


Chemical agents1

Chemical Agents

  • Phenolics – contain phenol or its derivatives

    • Disinfectant; intermediate to low level

  • Aldehydes

    • Formaldehyde

    • Disinfectant: high to intermediate level

  • Alcohols

    • Antiseptics and disinfectants; intermediate level


Chemical agents2

Chemical Agents

  • Halogens

    • Disinfectant and antiseptic; intermediate level

  • Heavy Metals

    • Disinfectant and antiseptic; low level

  • Soaps

    • Detergents derived from lipids

    • Antiseptic and disinfectants; low level

  • Gases

    • Disinfectant; high level


What can be used in our lab

What Can Be Used In Our Lab????

  • Soap

  • Disinfectant

  • Antiseptic

  • Sterile filtration

  • Bunsen Burner


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