The interaction of garlic with mediators of inflammation
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The Interaction of Garlic With Mediators of Inflammation. Prof. A. M. Vollmar Dr. V. M. Dirsch H - P Keiß. Overview. Interaction of garlic powders with NF- B Results Summary Interaction of garlic powders with cytokines Results Summary

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The Interaction of Garlic With Mediators of Inflammation

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The interaction of garlic with mediators of inflammation

The Interaction of Garlic With Mediators of Inflammation

Prof. A. M. Vollmar

Dr. V. M. Dirsch

H - P Keiß


Overview

Overview

  • Interaction of garlic powders with NF-B

    • Results

    • Summary

  • Interaction of garlic powders with cytokines

    • Results

    • Summary

  • Influence of garlic powders on endothelial NO-production

    • Assay setup

    • Results

  • Influence of garlic powders on apoptosis

    • Assay setup

    • Results

  • Outlook


Effect of garlic on nf b activity

Effect of garlic on NF-B activity

  • Neither spanish nor french garlic powders had an influence on TNF-induced NF-B activity

  • Garlic sulphur constituents don´t seem to interact with NF-B.


Effect of garlic on tnf liberation

Effect of garlic on TNF liberation

  • Garlic alone induces TNF liberation.

  • After LPS treatment garlic increases TNF release.


Effect of garlic on il 1 liberation

Effect of garlic on Il-1 liberation

  • Garlic alone shows weak Il-1 liberation.

  • Garlic increases Il-1 release in low concentrations.

  • In high concentrations GPE reduce LPS induced IL-1 levels.


Effect of garlic on il 10 liberation

Effect of garlic on Il-10 liberation

  • Garlic alone induces Il-10 liberation.

  • Garlic is able to increase Il-10 release after LPS stimulation


Influence of garlic powders on endothelial no production

NO

O2

DAF

DAF-2T

Influence of garlic powders on endothelial NO-production

  • Endothelial cells were incubated with garlic powder extracts for 20 h.

  • Supernatants were collected and subsequently incubated with Arginin,Ca2+-Ionophor A23187 and 4,5-diaminofluorescein.

  • Fluorescence of the supernatants were determined by spectrofluorimetry


Effect of garlic on no liberation

Effect of garlic on NO - liberation

Garlic extracts on HUVECs

1.40

1.20

1.00

0.80

Spain

x-fold control

0.60

France

0.40

0.20

0.00

µg/ml

Control

50

500

50

500

2000

2001


Mtt assay

Formazan

MTT

MTT-Assay

Cells used: S-Jurkat, Clone J16

Enzyms of the mitochondria membrane metabolise the yellow MTT to blue Formazan

> Assay to distinguish between dead and alive cells


Results i

Results I

  • DADS shows cytotoxic effects at 100 µM

  • AMSO2 and AMSO show weak cytotoxicity


Results ii

Results II

Garlic extracts show very weak cytotoxic effects


Trouble shooting

Trouble shooting

  • GPE showed strong bacterial and fungal contaminations

  • Different solvants had no effect on contaminations

  • Supplementation of antibiotics to media has cytotoxic effect on Jurkat cells.


Outlook

Outlook

  • Influence of garlic on PAI liberation

  • Interleukin 10 ??????


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