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Sir John Dalton (1766-1844)

Sir John Dalton (1766-1844). Low precision high accuracy. High precision low accuracy. R C A T. High performance liquid chromatography (HPLC). ( 1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve ( 4) Mixing vessel for delivery of the mobile phase ( 5) High-pressure pump

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Sir John Dalton (1766-1844)

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  1. Sir John Dalton (1766-1844)

  2. Low precision high accuracy High precision low accuracy

  3. RCAT

  4. High performance liquid chromatography (HPLC) (1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve (4) Mixing vessel for delivery of the mobile phase (5) High-pressure pump (6) Switching valve in "inject position” (6') Switching valve in "load position", (7) Sample injection loop (8) Pre-column (guard column) (9) Analytical column (10) Detector (i.e. IR, UV), (11) Data acquisition (12) Waste or fraction collector.

  5. 1,000-1,800 volts vs 180V (your wall socket) 220V (euro wall socket) 480V (3rd rail on the metro)

  6. Nano Electrospray Ionization nanoESI From http://www.mrc-mbu.cam.ac.uk/research/proteomics

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