1 / 21

Mechanical properties of chondrocytes from micropipette aspiration experiments

Mechanical properties of chondrocytes from micropipette aspiration experiments. Frank Baaijens 1 , Wendy Trickey 2 , Tod Laursen 3 , Leonidas Alexopoulos 2 and Farshid Guilak 2 1 Department of Biomedical Engineering, Eindhoven University of Technology

mingan
Download Presentation

Mechanical properties of chondrocytes from micropipette aspiration experiments

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mechanical properties of chondrocytes from micropipette aspiration experiments Frank Baaijens1, Wendy Trickey2, Tod Laursen3, Leonidas Alexopoulos2 and Farshid Guilak2 1 Department of Biomedical Engineering, Eindhoven University of Technology 2 Departments of Surgery and Biomedical Engineering, Duke University 3Department of Civil Engineering, Duke University

  2. Micro-pipette aspiration experiment PA FA

  3. Time dependent response

  4. Questions • Mechanical properties of chondrocytes • viscoelastic? • bi-phasic? • compressibility? • finite strains?

  5. Constitutive modeling

  6. Approximate analytical solution • Theret et al solution • final aspiration length • linear elastic, infinitesimal strains • incompressible • half-space approximation

  7. Deformation of chondrocyte Incompressible neo Hookean model

  8. Finite strains? Infinitesimal strains Haider, Guilak et al (2002) Finite strains

  9. Theret approximation: half space?

  10. Creep response

  11. Compressibility of chondrocytes

  12. Recovery experiment

  13. Compression Bi-phasic, compressible, viscoelastic

  14. Equilibration

  15. Recovery

  16. Results

  17. Discussion • Chondrocytes are compressible (ν~ 0.4) • Bi-phasic theory can not explain time dependent response of chondrocytes • Creep response due to viscoelastic behavior • Estimated time scales of PA and FA do not match: different parts of cells are involved in PA and FA

  18. Parameter estimation

  19. Theret approximation: bi-phasic?

  20. Compression and Recovery

  21. Creep recovery

More Related