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Flt3-L SCF

+. +. +. +. +. +. -. +. +. -. +. -. +. -. CD34 +. -. -. -. -. CD34 - lin - (week 4 of culture). a). 50. 6.0. b). 40. 4.5. 30. J1/2. JP1. JP2. CD34 mRNA (qPCR). Bcl-2 mRNA (qPCR). 3.0. JP. M. C. L. 20. Day 0. 1.5. CD3 +. Day 0. 10. 0. 0.0. Day 0. 9.

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Flt3-L SCF

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  1. + + + + + + - + + - + - + - CD34+ - - - - CD34-lin- (week 4 of culture) a) 50 6.0 b) 40 4.5 30 J1/2 JP1 JP2 CD34 mRNA (qPCR) Bcl-2 mRNA (qPCR) 3.0 JP M C L 20 Day 0 1.5 CD3+ Day 0 10 0 0.0 Day 0 9 15 Flt3-L SCF 12 6 9 Flt3-L,SCF IL-15 GATA-3 mRNA (qPCR) Id2 mRNA (qPCR) 6 Day 0 3 Day 0 Flt3-L,SCF IL-21 3 0 0 Flt3-L,SCF IL-15,IL-21 - - Flt3-L+SCF Flt3-L+SCF - - IL-15 IL-15 - - IL-21 IL-21

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