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《高级仪器分析技术》实验 PowerPoint PPT Presentation


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《高级仪器分析技术》实验. 食品与营养保健学院. 目录. 实验一:水的纯度测定及电导率仪的使用 实验二: 铁的分光光度计法测定 实验三: 琼脂糖凝胶电泳 实验 实验四: 高效液相色谱法测定茶叶中咖啡因. 《 高级仪器分析技术 》 实验一. 水的纯度测定及 电导率仪的使用. 一、电导率仪的使用. 液体中的电流流动. 在固体导体中,电流是通过 自由电子的移动 而产生的。 在液体导体中,电流是通过 自由离子的移动 而产生的。. 传输电流的离子物质被称为电解液。 电解液的实质是自由离子。 分子水解产生带正电的阳离子和带负电的阴离子。. 电导率.

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《高级仪器分析技术》实验

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  • S/mmS/mS/cmS/cm

    1S/m=1000mS/m

    =1000000S/m

    =10mS/cm

    =10000S/cm

  • =1/ 25 C


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  • -

  • -

  • -/

  • -/

  • -/

  • -/

  • -/

  • -//


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-

  • L

  • GSS=G


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: I = U / R

= U G

G = [S = 1/]

C [mS/cm, S/cm]:

C = G d/A

dA

= d/A [cm-1]

C = G

C


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ERxRmEmRm

KcellERmKcellEmEmEm

1.2.

3.4.


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  • 25C


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  • (anisotropic) 3 3


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?

  • T = ref (1 + (T-Tref))

  • with temperature coefficient :

  • =(T - ref)/ [ ref(T -Tref)]

  • ref= conductivity at reference emperature (e.g. 25 C)

  • T=conductivity atprocesstemperature

  • Tref= reference temperature (e.g. 25 C)

  • T= process temperature


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Gt = Gtcal{1 + (T-Tcal)}

Gt = C

Gtcal = C

Tcal =

= C

DDS-11C2%


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  • 30min

  • 1

    2

    3

  • 1RANGE


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  • 25250.1

  • JJ=K/KK


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  • CO2


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  • 2%/2525

  • 0.5S/cm


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  • 2


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  • 1.

    2.10

    3.

    4.


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  • 1.

  • 2.


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  • Na+K+Ca2+Mg2+CO32ClSO42


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  • 2540605


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722


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  • 1

    1

    2420 nm100%T0%T420 nm100.0%T


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  • 2

    1Mode

    2420nm

    3252.5


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4

5100%T

6


1 2 722

1.2.722


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  • d

    c


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    2.

    H3R

    Fe3+ H3RFe3+

    pH


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    pH9~11.5 NH3H2O-NH4Cl Fe3+


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    , Ca2+ Mg2+ Al3+, , F- NO3- PO43-Cu2+Co2+Ni2+Cr3+Fe2+

    420nm,


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    • 1.

      6 50 mL , 0.00 1.00 2.003.004.00 5.00mL0.1 mg/L 4 mL 10% NH4Cl2 mL 20%, (1+1), 1mL, , NH3-NH4Cl


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    A.Fe3+pH=10 pH9-11.531pHNH3-NH4Cl

    B.

    C.


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    420 nm, , 100%, , ,


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    1


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    • 2.

      5.00mL 50mL, , , ,

      -


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    2

    , , mg/L


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    • NH4Cl

    • 1

      2

      3


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    • Tise-leas

    • a)


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    • DNA

    • PCR


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    • D-3.6--L-


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    • DNA

    • DNADNA

    • DNA DNA DNA


    5449125


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    • 1. 1 TAE0.5TBE

    • 2.

      50%

    • 3.


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    • 4.60(EB)0.5ug/ml

    • 5. 35 mm

      (>60)

    • 6. 30 425


    5449125

    • Tris-EDTApH8.0,TAEE

    • Tris-(TBE)

    • Tris-(TPE)


    5449125

      • DNA

    • FF


    5449125

    • FF2.2

    • 0.5TBE300bpDNAFF4kbDNA0.5%-1.4%


    5449125

    • DNA

    • DNA


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    DNA

    • DNAUlogNN

    • >DNA


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    • DNA


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    DNA

    • >DNA>

    • DNA5-8V/cm


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    DNA


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    bio-rad


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    /


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    • 510TBETAETPETris- Tris-Tris-

    • TBE0.5TBE

    • 2g100ml 0.5TBE212


    5449125

    • 1mm

    • 2


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    • EB50-60(EB)0.5g /mL10mg/ml

    • 0.5TBE


    5449125

    • 10L DNA2L 6DNAtip

    • ,60-80V15v/cm40mA2cm

    • EB0.5g/mLEB20-25min


    5449125

    254nmEB


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    • DNA

    • DNA


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    1

    3

    9

    8

    4

    2

    7

    5

    10

    6

    12

    3

    4

    5

    6

    7

    8

    9

    10


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    • Shim-pack SCR-102H(8mmI.D300mmL.)

      12

      SCR-102H (6mmI.D.50mmL.)

      5mM p-

      0.8mL/min

      4045

    • 20mM Bis-tris

      5mM p-100 EDTA

      0.8mL/min

      polarity;+


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    1

    1.

    2.

    3.

    4.

    5.

    6.

    7.

    8.

    9.

    10.


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    1.

    2.

    3.

    4.

    5.

    6.

    7.

    8.

    9.

    10.

    10


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    1.

    2.

    3.

    4.

    5.

    6.

    7.

    8.

    10

    min.


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    1.

    2.

    3 .

    4.

    5.

    6.

    7.

    8.

    9.

    10

    10

    min.


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    1.

    2.

    3.

    4.

    5.

    6.

    7.

    8.

    9.

    10.

    10

    10


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    1.

    2.

    3.

    4.

    5.

    6.

    7.

    8.

    5uL


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    1.

    2.

    3.

    4.

    5.

    6.

    7

    10


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    1.

    2.

    3.

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    5.

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    1010


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    1.

    2.

    3.

    4.

    5.

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    10


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    :

    : --- UV/VIS

    OPA ---


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    PITC

    Column : Revered Phase Column (ODS)

    Reagent : OPA (Fluorescence)

    FMOC (Fluorescence)

    Fluorescamine (Fluorescence)

    DANS-Cl (Fluorescence)

    PITC (UV)


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    1. 200uL100mM

    100uL1M

    100uL,1

    2. 400uL

    3.

    0 .45um

    4. 4uL


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    1

    2

    3

    4

    5

    6

    7


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    1

    2

    3

    4

    5

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    8

    9


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    1

    2

    3

    4

    5

    6

    7

    8


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    • pH


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    • 70


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    • ()


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    • ,


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    • ()


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    .


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    • HPLC


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    /

    ODS


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    (nm)


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    • 0.45um


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    • 0.45um0.2um


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    • C8H10N4O2


    5449125

    1. 12501.620000.099mm6.251.51.181.05

    1


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    2.

    1 TC-C18 Agilent4.6 mm250 mm5 m-29:71280 nm()40 C1 mL/min10 L

    2 0.5 mg10 mL1 mL50 g/mL

    3 50 g50 mL95%95 C1.5 h50 mL1 mL50 mL


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    3.

    1 660 g/mL0.10.30.60.91.21.51.8 mL10 mL71

    1


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    2

    0.1 g/m-1.8 g/mA=21060C+56398r=0.9991


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    2 660 g/mL1.50.90.3 mL10 mL32

    2


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    3 660g/mL0.80.50.20.0mL1.0mL300g/mL3%=-/3

    3


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    4.


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    • 3


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    • C184.6 mm250 mm5 m-29:71280 nm()40 C1 mL/min, ,

    • 5


    5449125

    • 2000


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