From Shendure and Hanlee (2008) Nat. Biotech. 26:1135
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from Shendure and Hanlee (2008) Nat. Biotech. 26:1135. 454 platform – pyrosequencing. from Shendure and Hanlee (2008) Nat. Biotech. 26:1135. Pyrosequencing: several views. APS = adenosine phosphosulfate.

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From shendure and hanlee 2008 nat biotech 26 1135

from Shendure and Hanlee (2008) Nat. Biotech. 26:1135


From shendure and hanlee 2008 nat biotech 26 1135

454 platform – pyrosequencing

from Shendure and Hanlee (2008) Nat. Biotech. 26:1135


From shendure and hanlee 2008 nat biotech 26 1135

Pyrosequencing: several views

APS = adenosine phosphosulfate

Apyrase, a nucleotide degrading enzyme, continuously degrades unincorporated dNTPs and excess ATP. When degradation is complete, another dNTP is added.

from www.columbia.edu/cu/biology/courses/w3034/.../lec20_06.ppt


From shendure and hanlee 2008 nat biotech 26 1135

Solexa sequencing

from Shendure and Hanlee (2008) Nat. Biotech. 26:1135


From shendure and hanlee 2008 nat biotech 26 1135

Fig. 12-15, Garrett and Grisham, Biochemistry, 3rd ed.


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