CLONING DOGS AND CATS: WHERE DO WE STAND?
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CLONING DOGS AND CATS: WHERE DO WE STAND? Margaret V. Root Kustritz, DVM, PhD, DACT University of Minnesota. U NIVERSITY OF M INNESOTA. SFT-ACT 2008. Veterinary Medical Center. U NIVERSITY OF M INNESOTA. SFT-ACT 2008. Veterinary Medical Center. CLONING - Nuclear transfer

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UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CLONING

- Nuclear transfer

Nucleus from a single cell from the

donor is transferred into an oocyte

that has had its DNA removed, the

new oocyte fertilized and matured

in vitro, and the subsequent embryo

transferred into a recipient dam at

the morula or blastocyst stage.

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CLONING

- Nuclear transfer

- Any donor cell can be used

(skin biopsy from the ear)

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


After transfer of DNA, the single cell is

stimulated to begin division

IVM = in vitro maturation

Immature oocyte released

Low E2 / P4 environment

Cumulus cells must be present

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


SUCCESS RATES

- Cats

IVF and IVM - 50% development

to the blastocyst stage

Cats have been cloned (“Copy Cat”)

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


SUCCESS RATES

- Dogs

IVF and IVM - 8-37% development

to the blastocyst stage

One lab has produced cloned dogs.

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


“Snuppy” =

Seoul National University

+ puppy

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CONCERNS

- Disease of cloned animals

- Early senescence?

- Increased stillbirths

- Birth defects

- Embryo wastage

- Cat return rate 1.0%

- Dog return rate 0.1%

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CONCERNS

- Disease of cloned animals

- Early senescence?

- Increased stillbirths

- Birth defects

- Embryo wastage

- Cat return rate 1.0%

- Dog return rate 0.1%

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CONCERNS

- Embryo wastage*

- Cat return rate 1.0%

- Dog return rate 0.1%

- Cattle return rate 11.0%

- Mouse return rate 5.0%

- Goat and sheep return rate 3.0%

* Using adult cell for donor nucleus

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CONCERNS

- Expense

- Public perception

“Little Nicky”

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


CONCERNS

- Expense

- Public perception

“Rainbow” and her clone, “Copy Cat”

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


COMMERCIAL AVAILABILITY

- Projected cost = $50,000 / animal

- Genetic Savings and Clone

Website refers you to ViaGen –

“Note that ViaGen has no plans to

provide commercial dog or cat cloning

services.”

UNIVERSITY OF MINNESOTA

SFT-ACT 2008

Veterinary Medical Center


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