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Microscopes

Microscopes. Microscope History. Robert Hooke English scientist 1655 Used crude microscope to observe a thin slice of cork Saw boxes that reminded him of small rooms where monks lived. He decided to call them cells as that’s what they were called.

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Microscopes

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  1. Microscopes

  2. Microscope History • Robert Hooke • English scientist • 1655 • Used crude microscope to observe a thin slice of cork • Saw boxes that reminded him of small rooms where monks lived. He decided to call them cells as that’s what they were called. • He later observed cells in the stems and roots of plants

  3. Anton von Leeuwenhoek • 1665 • Dutch scientist • Used a microscope to view pond water • Discovered many living creatures he called “animalcules” (Little animals)

  4. A microscope is a tool used to look at things that are too small to see with the unaided eye. • Micro means small. • Scope means to look at. Microscopes allow us to see cells, which are the basic unit of life.

  5. Types of Microscopes • Simple Light • 1 lens • Energy source = light • Low magnification

  6. Types of Microscopes • Compound light • 2 + lenses • Energy source = light • Eyepiece lens = 10 • Objective = 4 • Total Magnification = 40 (Eyepiece lens X Objective)

  7. Types of Microscopes • Electron Microscope • Uses a beam of electrons • Magnify up to 500,000 times Types of Electron Microscopes • SEM = Scanning electron microscope • Surface • TEM = Transmission electron microscope • Inside

  8. Parts of a Microscope

  9. Important Terms • Magnification • Describes how much larger an image appears • Calculated by objective lens X ocular lens • Field of View • What can be seen when looking in a microscope • As magnification increases, field of view decreases

  10. How to Properly Use a Microscope • Place the microscope on a flat stable surface. • Plug the microscope in so that the cord does not stretch across the path that someone would walk through. • Turn the microscope on. • Make sure the objective lens is on low power. • Place your slide on the stage and secure with the stage clips.

  11. Proper Use Continued • Focus on low power using the coarse adjustment knob. • Change objective to high power by carefully rotating the nosepiece. • Focus on high power using the fine adjustment ONLY!!!

  12. Proper Storage • Objective lens switched to low power • Stage is clear • Cord is wrapped around the base • Covered

  13. Proper Way to Carry a Microscope • One hand on the arm and the other hand on the base.

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