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Personal Protective Equipment

Day II: Part 2 Sections: 12-13 Radiation Safety in the Working Environment. Personal Protective Equipment. PPE Gyrus. Scientists isolate ‘Common Sense’ Gene!. Personal Protective Equipment.

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Personal Protective Equipment

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  1. Day II: Part 2 Sections: 12-13Radiation Safety in the Working Environment

  2. Personal Protective Equipment

  3. PPE Gyrus

  4. Scientists isolate ‘Common Sense’ Gene!

  5. Personal Protective Equipment • The personal clothing of a worker must be of a type and in a condition which will not expose the worker to any unnecessary or avoidable hazards. • Worn by workers for protection against various hazards. WorkSafe BC 8.10 (1)

  6. Protective Clothing

  7. Protective Clothing • Loose fitting long pants • Lab Coat/Coveralls • Closed toed and heel shoes

  8. Eye Protection

  9. Protective Gloves

  10. Protective Gloves • Web site: http://www.bestglove.com

  11. Jewellery and Rad work Tears in your gloves + =

  12. Jewellery and Rad work Half – life of C-14 is 5730 years C-14 C-14 C-14 C-14

  13. Protective Footwear

  14. Personal Protective Equipment • Requires proper selection, training and supervision is ensure its effectiveness. • Is used when elimination, engineering or administrative controls are not practicable. • Must be administrated by knowledgeable persons.

  15. Safe Work Practices (cont’d)

  16. Use correctly!!!

  17. Maximum Distance of Beta Particles in Air • P32 - 6 m • Na22 -1.4 m • I131 -1.65 m • Ru86 -6.4 m

  18. Maximum Distance of Beta Particles and gamma radiation in Air • P-32 - 6 m • Na-22 - 1.4 m • I-131 - 1.65 m • Ru-86 - 6.4 m

  19. P-32 Protection P-32 Maximum range = 6m 260 mSV/hr 37 MBq P-32

  20. Wipe test equipment Remove contents (if applicable e.g. Fume hood) Inform HSE HSE gives clearance Maintenance proceeds T H E U N I V E R S I T Y O F B R I T I S H C O L U M B I A Laboratory Equipment Maintenance

  21. Shielding Sources Lead Metal Distributors 7220 Winston Ave. Burnaby 420-3731 Plexiglass GE Polymershapes 604 468 2112 Leaded Plexiglass ELLARD INSTRUMENTATION LTD Seattle 800-ELLARD1 http://home.earthlink.net/~eiltd

  22. Benefits of Non-Isotopic Methods No radiation exposure No specially designated equipment Simplified waste handling No special training No contamination control No record keeping Equal quality

  23. Non-isotopic Methods Labeling and Detection of Nucleic Acids DNA, RNA, Oligonucleotide probes In Situ Hybridization Sequencing

  24. Radioactive Biohazards CNSC Regulations Biosafety Regulations Public Health Agency of Canada Canadian Food and Inspection Agency CASE by CASE

  25. BIOHAZARD Replicate Dose Variability Spectrum of Effects RADIATION Constant Detectable Quantitative Half-life Radioactive Biohazards +

  26. Radioactive Biohazards Terminate radioactive biohazards with appropriate biocide i.e. phenol based compounds Do not Autoclave

  27. Radioactive Biohazards • Isotopes may be used in a Biosafety Cabinet: • Warning Symbol • Wipe Tests • Proper BSC techniques

  28. Plan to Succeed Expect to Fail Radioactive Biohazard or Biohazardous Radiation? • Plan ahead • Written Protocol • It WILL happen

  29. “Well....thanks a bunch, ‘Mister Who Needs a Written Protocol?’

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