DNA. A Technology for Change. Polymerase Chain Reaction (PCR) 1. Amplifies (makes additional copies) of target DNA sequence. Polymerase Chain Reaction (PCR) Steps CYCLE 1 Denature target DNA sequence (heat). Attach (anneal) 3’ and 5’ primers to opposite ends of targeted sequence.
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A Technology for Change
1. Amplifies (makes additional copies) of target DNA sequence
“Blunt end” cutting type
(Smal, Serratia marcescens)
“Sticky end” cutting type
(EcoR1, Escherichia coli)
of “cut” DNA piece Mixtures
Father Child Mother
E. Coli “conjugation” – gene/plasmid transfer “replicons”