DNA. A Technology for Change. Polymerase Chain Reaction (PCR) 1. Amplifies (makes additional copies) of target DNA sequence. Polymerase Chain Reaction (PCR) Steps CYCLE 1 Denature target DNA sequence (heat). Attach (anneal) 3’ and 5’ primers to opposite ends of targeted sequence.
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A Technology for Change
Polymerase Chain Reaction (PCR)
1. Amplifies (makes additional copies) of target DNA sequence
“Blunt end” cutting type
(Smal, Serratia marcescens)
“Sticky end” cutting type
(EcoR1, Escherichia coli)
Restriction Enzyme Examples
Gel Electrophoresis = Process for Separation
of “cut” DNA piece Mixtures
Electrophoresis Gel Data
Father Child Mother
Plasmids = transferable genetic elements, also called “replicons”
E. Coli “conjugation” – gene/plasmid transfer