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DNA. A Technology for Change. Polymerase Chain Reaction (PCR) 1. Amplifies (makes additional copies) of target DNA sequence. Polymerase Chain Reaction (PCR) Steps CYCLE 1 Denature target DNA sequence (heat). Attach (anneal) 3’ and 5’ primers to opposite ends of targeted sequence.

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A Technology for Change

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A technology for change

DNA

A Technology for Change


A technology for change

Polymerase Chain Reaction (PCR)

1. Amplifies (makes additional copies) of target DNA sequence


A technology for change

  • Polymerase Chain Reaction (PCR) Steps

  • CYCLE 1

  • Denature target DNA sequence (heat).

  • Attach (anneal) 3’ and 5’ primers to opposite ends of

  • targeted sequence.

  • Use DNA polymerase to complete replication of target sequence (by producing complementary

  • sides of denatured strands).

  • CYCLE 2

  • Repeat steps 1-3 above.

  • CYCLE 3…etc.


A technology for change

  • Restriction Enzymes

  • DNAase enzymes found in most cells

  • named for the source organism (e.g.,

  • ECOR1, isolated from E. coli bacteria)

  • enzymes that “cut” DNA in specific

  • patterns

“Blunt end” cutting type

(Smal, Serratia marcescens)

“Sticky end” cutting type

(EcoR1, Escherichia coli)


A technology for change

Restriction Enzyme Examples


A technology for change

Gel Electrophoresis = Process for Separation

of “cut” DNA piece Mixtures


A technology for change

Electrophoresis Gel Data

Father Child Mother


A technology for change

Plasmids = transferable genetic elements, also called “replicons”


A technology for change

E. Coli “conjugation” – gene/plasmid transfer


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