transcription cycle
Download
Skip this Video
Download Presentation
Transcription cycle

Loading in 2 Seconds...

play fullscreen
1 / 29

Transcription cycle - PowerPoint PPT Presentation


  • 125 Views
  • Uploaded on

Transcription cycle. Microcin j25. TRCF (mfd). GreA GreB. Rifampicin. Anti-  \'s. Activators. Transcription cycle. TRCF (mfd). Miller spread. Transcription Repair Coupling Factor (TRCF, mfd). !. Selby & Sancar (1993) Science 260 , 53. Mahdi et al. (2003) EMBO J . 22 , 724.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Transcription cycle' - melora


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
transcription cycle
Transcription cycle

Microcin j25

TRCF

(mfd)

GreA

GreB

Rifampicin

Anti-\'s

Activators

slide4

Transcription Repair Coupling Factor (TRCF, mfd)

!

Selby & Sancar (1993) Science260, 53.

slide5

Mahdi et al. (2003) EMBO J. 22, 724.

Chambers et al. (2003) Nucleic Acids Res. 31, 6409.

Selby & Sancar (1993) Science260, 53.

Selby & Sancar (1995) J. Biol. Chem. 270, 4882.

slide6

Rescue

• Transcript cleavage (stimulated by GreA/GreB)

• Slide forward

slide8

RecG

Singleton et al. (2001) Cell 107, 79

TRCF

Park et al. (2002) Cell 109, 757

slide9

Alexandra Deaconescu

Collaborators

Anna L. Chambers, Abigail J. Smith, Nigel J. Savery (University of Bristol, Bristol, UK)

Bryce E. Nickels, Ann Hochschild (Harvard Medical School)

slide10

Data collection and model refinement statistics for apo Mfd

Experimental electrondensity map

(after solvent modification)

Deaconescu et al. (2006) Cell124, 507.

slide13

APS NE-CAT-8BM

APS SBC-19ID

Deaconescu et al. (2006) Cell124, 507.

R/Rfree = 0.25/0.29 (3.2 Å resolution)

slide16

Singleton et al. (2001) Cell 107, 79

Deaconescu et al. (2006) Cell124, 507.

slide17

Truglio et al. (2002) EMBO J. 23, 2498

UvrB homology module, UvrA recruitment, and the role of D7

slide18

• necessary for RNAP binding (Selby & Sancar, 1995)

• binds RNAP b subunit 19-142 (Park et al., 2002)

slide21

A. L. Chambers, A.J. Smith, N. J. Savery

B. Nickels, A. Hochschild

Leu499

Smith & Savery (2005) Nucleic Acids Res. 33, 755

Park et al. (2002) Cell 109, 757

Park et al. (2002) Cell 109, 757

slide23

• TRCF - compact arrangement of structural domains linked by long, flexible linkers - appears ‘primed’ for large scale conformational changes (translocation module, UvrA binding surface).

? Nature and timing of TRCF conformational changes during the steps of TRCF-mediated transcription-coupled repair reaction? (recognition of a stalled RNAP; forward translocation of the RNAP to the transcription block; RNAP release and transcript termination; recruitment of the NER machinery to the site)

? Details of protein/protein interactions involving TRCF? (TRCF/RNAP and TRCF/NER machinery)

? Termination mechanism?

slide24

Anna L. Chambers, Abigail J. Smith, Nigel J. Savery (University of Bristol)

Bryce E. Nickels, Ann Hochschild (Harvard Medical School)

Chris Roberts, Jeff Roberts (Cornell University)

Chris Selby, Aziz Sancar (University of North Carolina, Chapel Hill)

N. Sukumar , M. Capel, S. Ealick (APS NE-CAT-8BM)

A. Joachimiak, S. Grinnell (APS SBC-19ID)

Darst Lab

Matthew Bick

Elizabeth Campbell

Linda (Madge) Champagnie

Alexandra Deaconescu

Raji Edayathumangalam

Deepti Jain

Valerie Lamour

William Lane

Fred Leon

Natacha Opalka

Georgia Patikoglou

Sheng Wang

Lars Westblade

Kelly-Anne Wilson

slide25

Bacterial transcription termination

Figure 1

A. Intrinsic

terminator

B. Rho

C. Mfd

slide27

Roadblock repression assay

(Chambers et al., 2003, Nucleic Acids Res. 31, 6409)

slide29

Epshtein & Nudler (2003) Cooperation between RNA polymerase molecules in transcription elongation, Science300, 801.

ad