Genomic prep did it work
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Genomic Prep: Did it work?. Is it DNA?. Ways to Tell: Microscope? Telescope? Some Dye? Spectroscopy Gel. Spectroscopy. How it works Absorbance DNA- 260nm Proteins- 280nm. What is Purity?. What does dirty DNA mean? Is there actual “dirt” on it?

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Genomic Prep: Did it work?

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Genomic prep did it work

Genomic Prep: Did it work?


Is it dna

Is it DNA?

  • Ways to Tell:

    • Microscope? Telescope?

    • Some Dye?

    • Spectroscopy

    • Gel


Spectroscopy

Spectroscopy

  • How it works

    • Absorbance

      • DNA- 260nm

      • Proteins- 280nm


What is purity

What is Purity?

  • What does dirty DNA mean?

    • Is there actual “dirt” on it?

  • Purity also refers to the presence of RNA and Protein.

    • Purity: Absorbance of DNA (260nm)/Absorbance of Protein (280nm)

  • Super important info:

    • An optical density (or "OD") of 1 corresponds to a concentration of 50 μg/ml for double-stranded DNA.


What is a good purity ratio

What is a good Purity ratio?

  • Guesses?

  • What does a ratio greater than ONE mean? Less than ONE?

  • Acceptable purity ratio: greater than 1.8

    • Meaning that the DNA is not contaminated protein.


Genomic prep did it work

Gel

  • What would a Gel look like if only Genomic DNA and dye were added?


Example

Example

From a Draft 2 paper


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