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Genomic Prep: Did it work?. Is it DNA?. Ways to Tell: Microscope? Telescope? Some Dye? Spectroscopy Gel. Spectroscopy. How it works Absorbance DNA- 260nm Proteins- 280nm. What is Purity?. What does dirty DNA mean? Is there actual “dirt” on it?

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Presentation Transcript
is it dna
Is it DNA?
  • Ways to Tell:
    • Microscope? Telescope?
    • Some Dye?
    • Spectroscopy
    • Gel
spectroscopy
Spectroscopy
  • How it works
    • Absorbance
      • DNA- 260nm
      • Proteins- 280nm
what is purity
What is Purity?
  • What does dirty DNA mean?
    • Is there actual “dirt” on it?
  • Purity also refers to the presence of RNA and Protein.
    • Purity: Absorbance of DNA (260nm)/Absorbance of Protein (280nm)
  • Super important info:
    • An optical density (or "OD") of 1 corresponds to a concentration of 50 μg/ml for double-stranded DNA.
what is a good purity ratio
What is a good Purity ratio?
  • Guesses?
  • What does a ratio greater than ONE mean? Less than ONE?
  • Acceptable purity ratio: greater than 1.8
    • Meaning that the DNA is not contaminated protein.
slide6
Gel
  • What would a Gel look like if only Genomic DNA and dye were added?
example
Example

From a Draft 2 paper

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