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Particle Measurement & Validation bv. Erkenningsnummer 3445-00. Peter van ’t Erve Project Manager International Service. Particle Measurement & Validation bv. Erkenningsnummer 3445-00. Introduction Basic stuff HEPA-filtration Types of cabinets Maintenance Criteria Disinfection

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Particle measurement validation bv

Particle Measurement& Validation bv

Erkenningsnummer 3445-00

  • Peter van ’t Erve

    • Project Manager

      • International Service


Particle measurement validation bv1

Particle Measurement & Validation bv

Erkenningsnummer 3445-00

  • Introduction

  • Basic stuff

  • HEPA-filtration

  • Types of cabinets

  • Maintenance

  • Criteria

  • Disinfection

  • HEPA-filter change

  • Miscellaneous


Particle measurement validation bv

Basic Stuff


Basic stuff basically a cabinet consists of

Basic stuffBasically a cabinet consists of :

  • The Body : Stainless Steel Epoxy coated steel

  • Prefilter : Eff. 80 – 90% acc. EN 779

  • HEPA-filters : Eff. 99,99% acc. EN 12469

  • Fan: To create the velocity

  • Electronics: To control the velocity and the alarm features

  • Accessories: on customer requirement


Basic stuff pre filters

Basic stuff Pre-filters

Filter classification according EN 779

Filter type

Pre-filters & Fine-filters

Filter class

G1

G2

G3

G4

F5

F6

F7

F8

F9

Am %

>65

65<80

80<90

90

-

-

-

-

-

Em %

-

-

-

-

40<60

60<80

80<90

90<95

95

  • Cheap glassfiber filter-material, to protect the expensive HEPA-filters

  • To be changed every year

  • Low cost exchange

  • Lifetime of a HEPA:

    without Prefilter appr. 6 years

    with Prefilter : appr. 12 years


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Basic stuff H.E.P.A. filters

  • HEPA = High Efficiency Particulate Air

  • ULPA = Ultra Low Particulate Air

The purpose of a HEPA-filter is to catch small particles.

Construction of a HEPA-filter:

  • Filter media (Glass fiber or similar)

    The media is pleated to a package of a certain size.

    The pleated media is glued together in a frame


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HEPA-Filtration


Hepa filtration efficiency

HEPA-Filtration: Efficiency

  • The efficiency of a HEPA-filter according EN 12469:

    99,99% when tested by means of a photometer.

100.000

particles

10 Particles


Hepa filtration sieve operation of a hepa

HEPA-FiltrationSieve operation of a HEPA


Hepa filtration interception impaction

HEPA-FiltrationInterception/impaction


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Types of cabinets


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  • Product Protection

  • Personal protection

  • Product and personal protection

Types of cabinets: General


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  • Crossflow

  • Downflow unit

  • Laminar Downflow

Types of cabinets: Product protection


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Class II B

Class II A

Class II A (Double exhaust)

HEPA-Filter

Pre-filter

Types of cabinets: Biological safety cabinets


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Inflow

Recirculation

Pressure plenum

Downflow

Exhaust

Types of cabinets: Biological safety cabinets

Flow pattern

30%

70%


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Maintenance


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Maintenance: General

  • Check on Pre-filter

  • Airflow velocity’s downflow & inflow

  • Filter efficiency test by means of a photometer or particle counter

  • Check on alarmsettings


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  • Product Protection

  • Personal protection

  • Product and personal protection

Maintenance: Control of Pre-filter

When do we need to change it?

Turn over one edge of the pre-filter,

If the down side is becoming grey, replace it.

Take care of contaminated areas !!


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Maintenance: Controlof air flow and velocity


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Maintenance: Airflow measurements (EN 12469)

Airflow velocity measurements

  • Downflow

  • Measurements have to be taken at

  • - 8 positions minimum,

  • 50 to 100 mm above the top edge of the front aperture.

  • Criteria:

  • All values have to be >0,25 and < 0,50m/s;

  • According manufacturer’s specifications;

  • No individual measurement should deviate more than 20% from the mean.


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1

2

3

Window

a

a

Working

space

H

b

c

b

c

L

1/8 L

4/8 L

7/8 L

Front view

Side view

Maintenance: Airflow measurements (EN 12469)

Airflow velocity measurements

Inflow

According Annex G you have to measure the velocity in the exhaust duct and calculate the volumetric rate of discharged exhaust air. With this air volume you can calculate the mean inflow velocity.

In practice it is much more easier to measure the inflow velocity in the front aperture.

Criteria:

Mean inflow velocity > 0,40 m/s and,

according manufacturer’s specifications.


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Maintenance: Airflow measurements (EN 12469)

Flow pattern

Tested using smoke

Inflow:

The direction of the inflow should be inward over the whole area of the front aperture.

Downflow:

The direction of the flow should be downwards without turbulence as a result of options or other obstructions in the working area.


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Maintenance: Control ofHEPA filter efficiency

Procedure:

Option 1.

Filtertest with a Particle Counter and a test aërosol.

Option 2.

Efficiency test with a Photometer and a test aerosol.

  • Criteria:

  • Tested with a Photometer a local penetration should be not more than 0,01%.

  • Related Standards:

  • IES-RP-CC006.2

  • ISO/CD 14644-3

  • EUROVENT 4/8


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Generating the test aerosol

(DOP, Emery 3004 , DEHS)

Referential measurement

(80-120 µgr / L)

100% measurement

Scanning probe

Photometer

Maintenance: Control ofHEPA filter efficiency

Downflow / Crossflow unit

Do not take a sample of the HEPA-filter !!!!!!!


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_

+

Generating the test aerosol

(DOP, Emery 3004 , DEHS)

100%

+

_

+

+

+

+

100% measurement

(80-120 µgr / L)

_

+

+

+

Probe

Scanningprobe

_

Photometer

_

_

_

Maintenance: Control ofHEPA filter efficiency


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Generating the test aerosol

(DOP, Emery 3004 , DEHS)

100% measurement

(80-120 µgr / L)

Scanningprobe

Photometer

Probe

100%

Maintenance: Control ofHEPA filter efficiency


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Maintenance: Alarm Settings (EN 12469)

Inflow

  • High Alarm

    • Create a high alarm by increasing the voltage of the fan and check whether there is a high alarm.

  • Low Alarm

    • Create a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm.

Downflow

  • High Alarm

    • Create a high alarm by increasing the voltage of the fan and check whether there is a high alarm.

  • Low Alarm

    • Create a low alarm by de-creasing the voltage of the fan and check whether there is a high alarm.

Window Alarm (If applicable)

Check whether there is an alarm if the window is not in a calibrated position.

Other alarmpoints

  • Check all other alarmpoints


Maintenance setting the alarms

Maintenance: Setting the alarms

  • Adjust velocity

  • Determine High alarm

  • (Vav + 20%)

High alarm

  • Determine Low alarm

  • (Vav - 20%)

Vav

Low alarm

  • Adjust alarmwindow to calculated alarmsettings

  • Adjust alarmwindow to calculated alarmsettings

    • First High alarm

  • Adjust alarmwindow to calculated alarmsettings

    • First High alarm

    • Then Low alarm

  • Make your alarm

  • window as big as possible


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Maintenance: Testpoints CA(B) / x and EF(B) / x cabinets

A: Aerosol supply

B: 100% measuring point (and test point for overall efficiency test

1st HEPA-filters on a CB and EFB)

This point might also be on top of the cabinet for a CA(B)

C: Scanning downflow HEPA-filter

D: Scanning exhaust HEPA-filter (overall efficiency test on a CA(B)


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Ducting: Ways of ducting

There are three ways to connect a safety cabinet to a duct!

  • Direct connection!

  • Direct connection with a by-pass!

  • Connection via a thimble!


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Externalfan

Anti Blow Back Valve

Airsupply

Ducting: Direct connection

  • Both cabinet and the external ventilator have to be switched on at the same time, this is done by a potential free contact (optional accessory) coming from the cabinet!

  • The anti blow-back-valve assures that no contaminated air can travel back into the cabinet!

  • The room pressure will fluctuate depending if the cabinet is switched on or off. However it is possible to have the air inflow adjusted to the amount of air that is extracted from the room!

  • Advantage: Higher level of safety!


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Externalfan

Anti Blow Back Valve

By-pass valve

Airsupply

Ducting: Bypass connection

  • The external ventilator works 24hrs a day!

  • When the cabinet is switched on the by-pass valve in the duct will open in order to extract the exhausted air from the cabinet.

  • When the cabinet is switched off the valve will close and will extract the same amount of air from the room!

  • The valve is also controlled by a potential free contact coming from the cabinet.

  • The anti blow-back-valve assures that no contaminated air can travel back into the cabinet!

  • Advantage: Higher level of safety and the room pressure will be constant at all time!


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Externalfan

Airsupply

Ducting: Via a Thimble

  • Effects on the roompressure:

  • When the cabinet is switched on the exhausted air will be extracted from the cabinet!

  • When the cabinet is switched off the exhausted air will be extracted from the room!

  • Advantage: No adjustments have to be made to the cabinet and the pressure in the room will be constant!


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What will be the effect on the alarm settings if the cabinet is connected on an exhaust duct work?

There are three possibilities:

  • There is no extra fan in the exhaust system.

Adjusting the alarms can be done by increasing or decreasing the downflow and inflow air velocity by means of the fan of the cabinet.

  • There is an extra fan in the exhaust system, but there is no link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan.

  • There is an extra fan in the exhaust system, with a link between the status (Day- or Stand-by position) of the cabinet and extra exhaust fan.


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Disinfection


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HEPA-filtered air

HEPA-filtered air mixed with product

Air mixed with fresh air and product

Contaminated air

Disinfection

  • Why desinfection:

- Microbiological Safety Cabinet is a Biohazard.

- All parts in the cabinet are a possible risk for

both personnel and environment when opened.

  • When desinfection:

- If repair needs to be done inside the cabinet.

  • With what can we desinfect a cabinet

- That depends on the product which was handled in the cabinet,

responsible for desinfection is the head of the laboratory.


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  • Clean Air techniek offers a so called FAS 2000 which

    is using Formalin for desinfection and Ammonia to

    neutralize the Formalin afterwards.

HEPA-filtered air

HEPA-filtered air mixed with product

Air mixed with fresh air and product

Contaminated air

FAS 2000

Disinfection

Using Formalin


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  • Depending on the size (volume) of the cabinet, Formalin is put in the can with the red cap and Ammonia is put in the can with the blue cap.

FAS 2000

Disinfection

Using Formalin


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  • In order to prevent Formaldehyde of getting into the

    laboratory, the cabinet has to be sealed off.

  • You can do this by sealing the cabinet with a plastic bag.

    Make sure that the bag is sealed to the floor.

Disinfection

Using Formalin


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HEPA-filter change


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HEPA-filter change

1st HEPA

  • First HEPA-filter on a Class IIB cabinet:


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HEPA-filter change

1st HEPA

  • Take of the pressurebar.

    (On older models you have to cut the silicon along the outer edge of the filter.)


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HEPA-filter change

1st HEPA

  • Seal the opening of the HEPA-filter in order

    to prevent parts to come out of the HEPA-filter


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HEPA-filter change

1st HEPA

  • Replace the HEPA by a new one and put the pressure

    bar back in it’s place.


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  • Take of the backpanel

  • Disconnect the fan and take it out

HEPA-filter change

Downflow HEPA


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  • Unloosen the pressure bars of the downflow plenum and the exhaust HEPA and take them out.

  • Lift the pressure plenum and take it out.

  • Take out the HEPA-filter.

Pressure bars

Plenum

HEPA-filter

HEPA-filter change

Downflow HEPA


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  • Put in a new HEPA-filter with the seal downwards and put everything back in it’s place.

HEPA-filter change

Downflow HEPA


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  • Lift the exhaustduct

  • Unloosen the pressurebars

    and take them out

  • Lift the pressure plenum

    and take it out

  • Take out the HEPA-filter

  • Put in a new HEPA-filter with an extra seal on one end.

  • Put everything back in it’s place

HEPA-filter change

Exhaust HEPA


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Criteria

Criteria


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Criteria Cabinet type EF / x (EN 12469)

- HEPA-filter efficiency downflow > 99,99%

- HEPA-filter efficiency exhaust > 99,99%

- Vaverage downflow velocity: 0,35 m/s.

  • Alarm for downflow velocity: Vaverage +/- 20% as where all individual measurements should be

  • > 0,25m/s and < 0,50m/s.

- Vaverage inflow velocity : > 0,30 m/s at the top of the working opening.

- Alarm for inflow velocity: < 0,28 m/s at the top of the working opening.


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Criteria Cabinet type EFB/ x (EN 12469)

- HEPA-filter efficiency downflow > 99,99%

- HEPA-filter efficiency exhaust > 99,99%

- HEPA-filter efficiency 1st HEPA > 99,99%

- Vaverage downflow velocity: 0,35 m/s.

  • Alarm for downflow velocity: Vaverage +/- 20% as where all individual measurements should be

  • > 0,25m/s and > 0,50m/s.

- Vaverage inflow velocity : > 0,30 m/s at the top of the working opening.

- Alarm for inflow velocity: < 0,28 m/s at the top of the working opening.


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  • Crossflow

  • Downflow unit

Criteria CLF x and DLF x

- According Federal Standard 209B

- HEPA-filter efficiency > 99,99%

- Vaverage downflow velocity: 0,35 m/s.

- Vaverage horizontal velocity: 0,45 m/s.

- Alarm : Vaverage - 20% and Vaverage +20% if applicable.


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Criteria Standards


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Video

VIDEO


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Test equipment

Test equipment


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Test equipment

Photometer

LIGHT STOP

CONE OF LIGHT

LIGHT SOURCE

PHOTO-

CONE OF

MULTIPLIER

DARKNESS

TUBE

3 WAY VALVE

VACUUM

UP

DOWN

FILTER

STREAM

STREAM


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Test equipment

Aerosolgenerator, generating a testaerosol DOP, EMERY 3004 or DEHS

ADJUSTING

KNOB

PRESSURE

REGULATOR

NITROGEN

INLET

FILTER

ORIFICE

AND

VALVE

SIGHT

GLASS

HEATER BLOCK

AEROSOL

RESERVOIR


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