Online Resource 1 List of species and NCBI accession numbers used for heat shock factor protein phylogenic and protein domain analysis.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Online Resource 1 List of species and NCBI accession numbers used for heat shock factor protein phylogenic and protein domain analysis
Online Resource 2 Sequence of HSE and CBF oligonucleotides used for EMSA. Nucleotides altered from the perfect HSE sequence are underlined. Over-hanging nucleotides used for end-fill labeling are indicated by lower case letters
Online Resource 3 Efficiency of hsf4 gene knockdown using four concentrations of MO. Total numbers of embryos injected with hsf4-MO and hsf4 5bpmm-MO as well as uninjected and danieau injected controls are listed. The percentage of embryo survival 48 hours after injection is also listed. Efficiency of the MO injections in surviving embryos was determined by calculating percentage of embryos that displayed strong flouresceine fluorescence. The number of embryos containing flouresceine and showing non-specific deformities at each MO concentration was tallied to determine the optimal MO concentration for injection