Average % primer efficiency:

1. 100 75 Relative mRNA expression 50 25 0 Tspan5 Tspan10 Tspan14 Tspan15 Tspan17 Tspan33 Average % primer efficiency: 92.0 111 94.3 96.7 98.6 110 Supplementary Figure IV. Validation of mouse TspanC8 tetraspanin RT-PCR primers. The relative mRNA levels of TspanC8 tetraspanins were measured by RT-PCR from a mixture of cDNAs from mouse brain, heart, kidney, liver, lung, muscle and thymus tissue. Standard curves were used for each primer and primer efficiencies obtained using the MxPro RT-PCR software from Stratagene (error bars represent the standard error of the mean from two experiments, each done in duplicate).