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WedNesday April 8 th 2014. QU: What does it mean to recombine? **Chapter 13 Test April 17 th 2014** OBJ: Bio-Technology “The life of an eyelash is about 5 months.”. DNA TECHNOLOGY: Biotechnology. Recombinant DNA Process. Recombinant DNA.

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WedNesday April 8 th 2014

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Wednesday april 8 th 2014

WedNesday April 8th 2014

  • QU: What does it mean to recombine?

    **Chapter 13 Test April 17th 2014**

  • OBJ: Bio-Technology

    “The life of an eyelash is about 5 months.”

Dna technology biotechnology

DNA TECHNOLOGY:Biotechnology

Recombinant DNA Process

Recombinant dna

Recombinant DNA

  • Some of the most important techniques used in biotechnology involve making recombinant DNA molecules

Recombinant dna1

Recombinant DNA

  • Recombinant DNA molecules are pieces of DNA that have been combined from pieces taken from more than one source of DNA

    • Often one of the sources is a Plasmid, the other is contains a gene we want



  • Small circular DNA molecules that can be in cells.

    • Often contain genes for resistance to antibiotics.

      • They are also used for human benefit.

Restriction enzymes

Restriction Enzymes

  • Possible because of special enzymes that cut DNA: called RESTRICTION ENZYMES

Dna scissors intro to restriction enzymes

DNA Scissors: Intro to Restriction Enzymes

  • Restriction enzymes recognize and bind to specific DNA sequences and cut the DNA at or near this site.

Restriction sites

Restriction Sites

  • These recognition cleave sites are called restriction sites.

  • Each different restriction enzyme has its own type of site.

Restriction enzymes1

Restriction enzymes

  • Can distinguish between the DNA normally present in the cell and foreign DNA, such as infecting bacteria virus DNA

  • The enzymes defend the cell from invasion by cutting the foreign DNA into pieces so it doesn’t work.

Restriction enzymes2

Restriction Enzymes

  • Restriction enzymes appear to be made exclusively by prokaryotes. (bacteria)

    • So bacteria is vital to DNA Technology because it makes the restriction enzymes.

Thursday april 10 th 2014

Thursday April 10th 2014

  • QU: What are restriction enzymes?

  • OBJ: Restriction Enzyme Lab, Notes

    “Rats can swim for half a mile (0.8 km) without rest, and can tread water for three straight days.”

**Chapter 13 Test April 17th 2014**

Recombinant dna2

Recombinant DNA

  • Identify the desired gene

  • Cut the gene out of the DNA Strand with restriction enzymes

  • Cut the plasmid with the same restriction enzymes and added the desired gene to the plasmid by matching the sticky ends

  • Place plasmid in the bacteria

    • Allow the bacteria to replicate and clone the gene

      • This replicates the desired gene

      • Protein synthesis creates the desired protein

Genetic modification

Genetic Modification

  • Genetically Modified Organism (GMOs)

    • Any organism that has acquired genes by artificial means.

    • Replaces traditional plant breeding

    • The goal is to make it bigger, better, more desirable organisms

How gmos are created

How GMOs are created?

  • Animals

    • Injecting a gene into a fertilized egg and then returning that egg to a uterus where it can develop

  • Plants

    • Introducing recombinant plasmid into a plant cell and letting it grow into a plant.

Friday april 11 th 2014

Friday April 11th 2014

  • QU: Describe Recombinant DNA?

    Journal Due Today

    Chapter 13 Test April 17th

  • OBJ: Recombinant DNA Lab

    “The Giant Burrowing Frog doesn't croak. It hoots like an owl.”

Monday april 14 th 2014

Monday April 14th 2014

  • QU: Why is it important to be able to replicate our plasmid?

    *Chapter 13 Test April 17th*

  • OBJ: DNA Fingerprinting Notes and Activity

    “The world's smallest rodent is Pygmy Jerboa - measuring only a couple of inches in length - and can run as fast as a horse.”

Dna fingerprinting

DNA Fingerprinting

  • How is DNA Fingerprinting done?

  • Scientists use the variable regions in our DNA to generate a DNA profile of an individual, using samples from blood, bone, hair, and other body tissues and products.

Wednesday april 8 th 2014

  • In criminal cases, this generally involves obtaining samples from crime-scene evidence and a suspect, extracting the DNA, and analyzing it for the presence of a set of specific DNA regions (markers).

  • Forensic scientists compare these DNA profiles to determine whether the suspect's sample matches the evidence sample.

Wednesday april 8 th 2014

To get the DNA fingerprint:

  • All of the DNA is broken into pieces at certain locations.

    • Done by restriction enzymes

  • The DNA pieces are placed at the top of a special gel held in a frame.

    • An electric current is applied to the gel. The current separates the DNA into bands of identical pieces. This process is called electrophoresis.

    • This electric current pulls the negatively charged DNA to the positive end of the gel.

Last notes on dna fingerprinting

Last Notes on DNA Fingerprinting

  • DNA Fingerprinting moves DNA by applying an electric current during electrophoresis.

    • This electric current pulls the negatively charged DNA to the positive end of the gel.

Dna fingerprinting1

DNA Fingerprinting

  • The band pattern is transferred to a nylon membrane containing a radioactive chemical. The chemical makes the bands show up clearly.

Why it is done

Why It Is Done

DNA fingerprinting is done to:

  • Determinewho a person's parents or siblings are.

    • This test also may be used to identify the parents of babies who were switched at birth.

  • Solve crimes (forensic science).

    • Blood, skin, or other tissue left at the scene of a crime can be analyzed to help prove whether the suspect was or was not present at the crime scene.

  • Identify a body.

    • This is useful if the body is badly decomposed or if only body parts are available, such as following a natural disaster or battle.

Wednesday april 16 th 2014

WedNesday April 16th 2014

  • QU: Describe how DNA fingerprinting works.

    *Chapter 13 Test Tomorrow*

    Chapter 13 Review Due tomorrow

  • OBJ: DNA Fingerprinting, Review for Ch 13 Test

    “The normal energy used by our brain is 0.1 calories per minute, and could go up to 1.5 during activities such as puzzle-solving.”

Thursday april 17 th 2014

Thursday April 17th 2014

  • QU: How did you study for this test?

    *Turn your chapter review into the HW bin*

  • OBJ: DNA Technology Test, Organize Binder

    “The normal energy used by our brain is 0.1 calories per minute, and could go up to 1.5 during activities such as puzzle-solving.”

Binder organization ch 13

Binder Organization: Ch 13

  • DNA Technology Hand Written Notes

  • Restriction Enzyme Lab

  • Recombinant DNA Lab

  • Sub work (Book Work)

  • Quiz #1 DNA Technology

  • DNA Fingerprinting worksheet

  • DNA Technology

  • Chapter 13 Review

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