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Bioinformatics Resources Provided by WV-INBRE

Bioinformatics Resources Provided by WV-INBRE. Mary E. Davis, Ph.D. Bioinformatics Core Director. Outline. Overview of WV-INBRE Technology supported Agilent microarray Illumina HiSeq1000 (Next Generation Sequencing) Bioinformatics analysis resources supported

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Bioinformatics Resources Provided by WV-INBRE

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  1. Bioinformatics ResourcesProvided by WV-INBRE Mary E. Davis, Ph.D. Bioinformatics Core Director

  2. Outline • Overview of WV-INBRE • Technology supported • Agilent microarray • Illumina HiSeq1000 (Next Generation Sequencing) • Bioinformatics analysis resources supported • Ingenuity Pathways Analysis (IPA) • Partek Genomics Suite • (Galaxy)

  3. Technology • Microarray • Agilent (Marshall Univ—WV-INBRE) • Affymetrix (WVU Neuroscience CoBRE) • Next Generation Sequencing • IlluminaHiSeq (Marshall Univ—WV-INBRE) • IlluminaMiSeq(WVU Neuroscience CoBRE)

  4. Bioinformatics Tools: Ingenuity

  5. IPA Function Result

  6. IPA: Effects on Function

  7. IPA: Upstream Regulators

  8. IPA: Canonical Pathways

  9. IPA: Gene Annotation

  10. Example of Findings in Gene Annotation Ingenuity scientists read literature and enter results using a structured vocabulary. This, in combination with disease and function ontologies, is basis for relationships in pathways.

  11. IPA: Pathways

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