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Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells

10 th AOTA Congress BALI, INDONESIA, 2012. Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells. Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu, China Contact: zhangli@jsinm.org. ?. Curcumin. Thyroid cancer. Curcumin.

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Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells

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  1. 10th AOTA Congress BALI, INDONESIA, 2012 Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu, China Contact: zhangli@jsinm.org

  2. ? Curcumin Thyroid cancer Curcumin • An acitve polyphenol derived from the plant turmeric • low toxicity • anti-inflammatory • antioxidant • antiangiogenic • antitumour Chemical structure of curcumin

  3. Apoptosis and Autophagy Apoptosis –type I programmed cell death Autophagy- type II programmed cell death - a self-degradative process - cope with stresses - required in apoptotic-deficient cells Three types of autophgay: • Microautophagy • Chaperone-mediated autophagy • Macroautopahgy

  4. Part I Crucumin suppresses BCPAP cells growth

  5. Effects of curcumin on cell viability A B

  6. Effects of curcumin on cell morphology

  7. PI/Hoechst 33342 staining A B

  8. Part II Curcumin induces apoptosis in BCPAP cells

  9. Effects of curcumin on pro-apoptotic and anti-apoptotic proteins A B

  10. Effects of curcumin on loss of mitochondrial membrane potential (MMP)

  11. Caspase-dependent apoptosis in curcumin-treated cells

  12. Part III Curcumin induces autophagy in BCPAP cells

  13. Induction of autophagy in curcumin-treated cells A B The FASEB JournalJune 2003vol. 17 no. 9 1165-1167

  14. C Curcumin (mM) 0 50 RFP-LC3 Induction of autophagy in curcumin-treated cells

  15. Effects of curcumin on the phosphorylation of AKT pathway A B

  16. Effects of curcumin on the phosphorylation ofMAP kinases

  17. Curcumin selectively induces autopagy in tumor cells A B

  18. Curcumin seletively induces cancer cell death A B

  19. Dynamic distribution of curcumin in BCPAP cells Enlarged 6 h 12 h 24 h

  20. Effects of curcumin on DNA conformation A B

  21. Conclusions • Curcumin induces cytotoxicity of BCPAP cells. • Induction of  apoptosis  and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin . • Curcumin inhibited AKT pathway and activated mitogen-activated protein kinase (MAPK) signaling thereby inducing autophagy.

  22. Acknowledgements Jiangsu Institute of Nuclear Medicine Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei China Medical University Guan Haixia Nangjing University Sun Yang Grants • National Natural Science Foundation of China (No. 30870727) • Natural Science Foundation of Jiangsu Province (No. BK2010156) • Science and Research Foundation of Health Bureau of Jiangsu Province (No. H2008031) Thank you for your attention!

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