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Lab #5 Photosynthesis S61

Lab #5 Photosynthesis S61. Procedures: www.kabt.org/2008/09/29/video-on-sinking-disks-for-the-floating-leaf-disk-lab/. AP LAB: Photosynthesis. Procedures: 1. Prepare 300mL of 0.2% bicarbonate solution for each experiment.(CO 2 )

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Lab #5 Photosynthesis S61

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  1. Lab #5 PhotosynthesisS61 • Procedures: • www.kabt.org/2008/09/29/video-on-sinking-disks-for-the-floating-leaf-disk-lab/

  2. AP LAB: Photosynthesis • Procedures: • 1. Prepare 300mL of 0.2% bicarbonate solution for each experiment.(CO2) • 2. Pour the bicarbonate solution into a clear plastic cup (100 mL). Label cup “w/CO2” • 3. Fill the second cup (100 mL) with water. Label this “w/o CO2” • 4. Add 1 drop of diluted liquid soap to each cup. • 5. Using a hole puncher, cut 10 or more uniform leaf disks for each cup. • 6. Obtain 2-10cc syringe and place 10 leaf disks inside the each barrel. Replace the piston, be careful not to crush the leaf disks. Push in the plunger until only a small volume of air and leaf disk remain.

  3. 7. Pull a small volume (5cc) of sodium bicarbonate + soap from your cup into one syringe and a small volume (5cc) of water + soap from your cup into the other syringe. 8. Make a vacuum in the plunger to draw the air out of the leaf tissue. (This is the most difficult step to master). • 9. Pour the disks and solution from the syringe into the appropriate cups. • 10. Place both cups under the light source and start the timer. After every minute, count the number of disks floating. Continue until all the disks are floating. • 11. Record your data • 12. NOW: test 2 other variables and collect data.

  4. Lab #5 Photosynthesis • Questions???? • 1. What variables of the plant leaves might affect the net rate of photosynthesis? • 2. What environmental factors might affect the rate of photosynthesis?

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