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Control (CTL). +TGF- β. BRI-JM01/ NMuMG cells. lectin affinity enrichment (WGA). trypsin. trypsin. nano-LC-MS analysis (Q-TOF). x 3 biological repetitions. (3 MS replicates/sample). Quantitation of peptide elution profiles. Quantitation of peptide elution profiles.

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Presentation Transcript

Control (CTL)

+TGF-β

BRI-JM01/

NMuMG

cells

lectin affinity

enrichment

(WGA)

trypsin

trypsin

nano-LC-MS analysis

(Q-TOF)

x 3 biological

repetitions

(3 MS replicates/sample)

Quantitation of peptide elution profiles

Quantitation of peptide elution profiles

Matching of peptides across data files

Determination of differentially expressed

peptide peaks

MatchRx software

Identification of differentially-expressed

peptides by MSMS

(1) include list (Q-TOF)

(2) auto-MSMS (LTQ/Q-TOF)

Determination of differentially expressed PROTEINS


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