Enzyme catalysis with catalase
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Enzyme Catalysis with Catalase. Key Notes From Lab Handout. Enzyme: Catalase (from beef liver) Substrate: H 2 O 2 (hydrogen peroxide) Stop Reaction: use sulfuric acid (H 2 SO 4 ) Quantify Amt. of H 2 O 2 left: titrate in KMnO 4 (potassium permanganate)

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Enzyme Catalysis with Catalase

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Enzyme catalysis with catalase

Enzyme Catalysis with Catalase


Key notes from lab handout

Key Notes From Lab Handout

  • Enzyme: Catalase (from beef liver)

  • Substrate: H2O2 (hydrogen peroxide)

  • Stop Reaction: use sulfuric acid (H2SO4)

  • Quantify Amt. of H2O2 left: titrate in KMnO4

    (potassium permanganate)

  • Baseline: Starting total amount of H2O2

  • FOUR ways enzyme activity can be altered:

    • Salt concentration (salinity)

    • pH (acid/base concentration)

    • Temperature

    • Activators & Inhibitors


Lab 3 enzyme catalysis w catalase

Lab #3- Enzyme Catalysis w/Catalase

Three Parts to the lab:

  • Establish Baseline Amount of H2O2

  • Uncatalyzed Decomposition of H2O2

  • Time Trials w/Catalase to determine Rxn rate

  • Procedure:

    • 10 ml H2O2 in a beaker

    • 1.0 ml (H2O or Catalase)

    • 10 ml 1 M H2SO4

    • Mix well

    • Take a 5 ml sample and titrate in KMnO4

    • Read Initial and final measurements on buret

    • Record Data


Enzyme catalysis with catalase

Sample Data Set I


Sample data ii

Sample Data II


Conclusion questions to consider

Conclusion Questions to Consider

  • Determine the amount of H2O2 in moles using the chemical equation on pg. 22 and your amount of H2O2 after 180 sec.

  • From Question #6, Choose two factors that can affect the rate of enzyme-catalyzed reaction of Catalase. Draw a predicted graph for each reaction.

  • Name two organisms that could be used to determine the reaction rate of an enzyme.

  • List two major sources of error and one suggestion on how to improve the lab. What would you do differently?


Enzyme catalysis with catalase

You can use either Set I or Set II if your data did not come out right!!!

When doing your analysis be sure to use good data especially if your values did not come out similar to what set I & II show!!!


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