1. Supplemental Figure 2. Coinfiltration of the CaM antagonist W7 with avirulent Pst avrRpt2+ does not affect the pathogen-associated long-term sustained cytosolic Ca2+ elevation. Leaves of aequorin-expressing plants were infiltrated with the avirulent pathogen (dark line) or coinfiltrated with the pathogen and CaM antagonist W7 (200 µM) (light line). This experiment is similar to that shown in Figure 3, except luminescence was recorded for 3 h prior to initiating Ca2+ release. We find here (also data not shown) a different avirulent pathogen-associated cytosolic Ca2+ ‘signature’ with Pst with avrRpt2+ than that reported by Grant et al. (2000) with aequorin-expressing WT Arabidopsis plants inoculated with Pst harboring either the avr genes avrRpm1 or avrB. We note a long sustained Ca2+ elevation occurring with Pst avrRpt2+ (beginning at ~45 min after inoculation, as shown above) without any rise to a level above that of the first peak (i.e. occurring at ~ 8 min in the experiment shown above). However, in all cases tested, we never see a reduction in cytosolic Ca2+ elevation occurring when leaves are coinfiltrated with pathogen and W7, as is shown in this experiment. Note that in this experiment, W7 application resulted in an increase in the ~8 min peak (highlighted by the arrow); a result similar to that shown in Figure 3. The significance of the results shown in this figure to the work in this report is that W7 application with avr pathogen does not impair pathogen-associated cytosolic Ca2+ elevations.