Part i purification of igy
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Part I Purification of IgY. BTCI – Field Trip Steve Harris SPASH – Biotechnology I. Protocol. Label two Eppendorf tubes. Fold paper towel into a weigh boat. Crack an egg over a beaker and place yolk on paper towel. Transfer 2ml of egg yolk into a tube of 8mls precipitation buffer A.

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Part I Purification of IgY

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Part i purification of igy

Part IPurification of IgY

BTCI – Field Trip

Steve Harris

SPASH – Biotechnology I


Protocol

Protocol

  • Label two Eppendorf tubes


Part i purification of igy

  • Fold paper towel into a weigh boat.


Part i purification of igy

  • Crack an egg over a beaker and place yolk on paper towel.


Part i purification of igy

  • Transfer 2ml of egg yolk into a tube of 8mls precipitation buffer A.


Part i purification of igy

  • Mix by Inversion for 2min


Part i purification of igy

  • Filter mixture

  • After filtering remove gauze and discard.


Part i purification of igy

  • Add 1ml of filtered egg yolk into one of your micro centrifuge tubes.


Part i purification of igy

  • Centrifuge for 10min.


Part i purification of igy

  • Remove 500µl of sup(supernatant) from fist tube into your second tube.


Part i purification of igy

  • Add 100µl precipitation buffer B


Part i purification of igy

  • Mix by Inversion for 2min

Like this again but with small tube.


Part i purification of igy

  • Centrifuge again for 10min.


Part i purification of igy

  • Remove supernatant without disturbing pellet

  • Continue using pipette to remove all supernatant

Pellet


Part i purification of igy

  • Add 1ml of 1xPBS in 500µl aliquots to the pellet and resuspend pellet by pipetting.


Part i purification of igy

  • Resuspending by vortexing.


Part i purification of igy

STOP

SWITCH BACK

TO PART II


Mr h say s

Mr. H say’s.

Great Results

I love

SPASH and BTCI


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