DNA Microarray Technology. Nucleic acid hybridization Construction of (human) genomic and cDNA libraries Polymerase chain reaction (PCR) Expression microarrays Work-flow of microarray experimentation. Nucleic Acid Hybridization.
Read the entire paper and get a general idea what the paper is about.
It is important to know the 3 different types of microarrays (cDNA, short and long oligos).
There are two important parts of Table 1 labeled as "Advantages" and "Disadvantages" respectively.
The sections "Accuracy of array experiments", "Which platform is best?", "Comparison of microarray expression platforms", and "Outlook and conclusions" should be read carefully and you should know the key points the author makes in those sections. However, I will not expect from the students to know the details from those sections. The "Highlights" box on pg. 500 could serve as a guide of what is important.
- Housekeeping genes
- Spiked-in controls
- Global median
- Total intensity
- LOWESS correction
Formulate the question
Interpretation of results
The convergence of DNA hybridization.
Solid surface DNA capture.
The array that contains immobilized nucleic acid sequences or target.
A detection system that records and interprets the hybridization signal.
Markers for Mendelian diseases with complex traits efficiently. Personalized drug development.
Studying the Loss of heterozygosity (LOH). LOH is a form of allelic imbalance. Has the potential in cancer diagnostics as LOH is a prominent characteristic of most human cancers.
High-throughput assays technologies
DNA• Polymorphism• SNP arrays
• Mutation• CGH arrays
• Loss of Heterozigosity
RNA • Expression levels• Microarrays
Protein• Relative abundance• Mass Spectrometry
• Modification• ChIP2chip
… ACGGCTAA …
… ATGGCTAA …
C(opy)N(umber) and LOH
(mutations, heterogeneity, …).
Measuring genetic variation