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Figure 1. 50 ng/ml NGF. 5 ng/ml NGF. A. NGF + SB203580. Control. NGF + SB202190. NGF. B. 8. 6. p38 MAPK phosphorylation (Fold stimulation). 4. 2. 0. 10. 20. 30. Time (min). P-p38 T-p38. 5 ng/ml NGF. P-p38 T-p38. 50 ng/ml NGF. Time (min). 0. 1. 3. 5. 10. 15.

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slide1

Figure 1

50 ng/ml NGF

5 ng/ml NGF

A

NGF +

SB203580

Control

NGF +

SB202190

NGF

B

8

6

p38 MAPK

phosphorylation

(Fold stimulation)

4

2

0

10

20

30

Time (min)

P-p38

T-p38

5 ng/ml

NGF

P-p38

T-p38

50 ng/ml

NGF

Time (min)

0

1

3

5

10

15

20

30

C

8

6

p38 MAPK

phosphorylation

(Fold stimulation)

4

2

0

1

10

100

1000

Log [NGF] (ng/ml)

P-p38

T-p38

0

5

10

30

50

100

300

1

NGF (ng/ml)

slide2

Figure 2

Control

5 ng/ml NGF

50 ng/ml NGF

P-p38

T-p38

NGF

PTX

Basal

5 ng/ml NGF

6

PTX

*

*

*

*

4

*

*

2

0

P-p38

T-p38

p38 MAPK phosphorylation

(Fold stimulation)

6

PTX

*

*

*

4

#

#

#

2

0

P-p38

T-p38

Gz

Gi2

GoA

Gi2/z

GoACI

Control

A

8

*

*

6

#

p38 MAPK phosphorylation

(Fold stimulation)

#

4

2

0

P-p38

T-p38

PTX

B

C

slide3

Figure 3

A

IP

IP

Lysate

Lysate

TrkA

TrkA

IgG

IgG

WB

P-TrkA

Gi2

Gz

Go

NGF

NGF

B

6

*

PTX

4

2

0

P-p38

T-p38

p38 MAPK phosphorylation

(Fold stimulation)

6

4

#

2

0

P-p38

T-p38

PTX

Gi2

Gi2GT

slide4

Figure 4

Basal

5 ng/ml NGF

5 ng/ml NGF + inhibitor

A

B

*

14

*

6

ERK

JNK

*

*

12

*

*

MAPK

phosphorylation

(Fold stimulation)

p38 MAPK

phosphorylation

(Fold stimulation)

4

10

8

#

2

#

#

4

0

0

P-MAPK

T-MAPK

P-p38

T-p38

NGF

NGF

NGF

U0126

U0124

U0124

U0126

Control

Control

Control

SP600125

SP600125

slide5

Figure 5

A

B

Basal

5 ng/ml NGF

5 ng/ml NGF + inhibitor

6

*

*

*

*

*

*

*

*

*

*

*

4

p38 MAPK phosphorylation

(Fold stimulation)

#

#

2

#

0

P-p38

T-p38

W7

PP1

PP2

PP3

NGF

NGF

KN62

AG490

U73122

U73343

SU6656

Control

Control

AG1295

Calphostin C

Staurosporine

C

3

*

2

#

Src phosphorylation

(Fold stimulation)

1

0

P-Src

T-Src

PTX

slide6

Figure 6

NGF

Trk A receptor

GIPC1

GAIP

Gi2

Src

G

Src

Gi2

Crk

Rho

GEF

C3G

Ras

Rac/

cdc42

Rap1

RhoA

B-Raf

MEKK1-4

MLKs

MEK1/2

MKK4

MKK3/6

p38 MAPK

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