Holographic optical trapping for live cell structuring
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Holographic optical trapping for live cell structuring ECEN 2010 Robert McLeod, 4/5/13. Question. Porous scaffold design for tissue engineering , Scott J. Hollister, Nature Materials 4, 518 - 524 (2005). http://www.memsjournal.com/2010/07/mems-for-neuroscience-research-applications.html.

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Holographic optical trapping for live cell structuring ECEN 2010 Robert McLeod, 4/5/13

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Holographic optical trapping for live cell structuring ecen 2010 robert mcleod 4 5 13

Holographic optical trapping for live cell structuring

ECEN 2010

Robert McLeod, 4/5/13


Question

Question

Porous scaffold design for tissue engineering, Scott J. Hollister, Nature Materials 4, 518 - 524 (2005)

http://www.memsjournal.com/2010/07/mems-for-neuroscience-research-applications.html

http://en.wikipedia.org/wiki/Organ-on-a-chip

http://www.nextnature.net/2007/01/how-to-print-an-organ/

Engineered 'jellyfish'

Nature 487, 408 (26 July 2012)

Can we add living tissue to 3D printing?


Optical momentum as a micromanipulator

Optical momentum as a micromanipulator

http://en.wikipedia.org/wiki/Optical_tweezers


Trapping forces in the rayleigh limit

Trapping forces in the Rayleigh limit

http://www.unc.edu/jacoblab/optical%20tweezers.htm

http://en.wikipedia.org/wiki/Stokes%27_law


Iterative trap calculation on gpu

3 iterations, 8 ms compute/load time

5 iterations, 13 ms compute/load time

Iterative trap calculation on GPU

mirror

10 iterations, 27 ms compute/load time

20 iterations, 54 ms compute/load time

Dynamic holographic optical tweezers, Curtis et al., 2002

SLM: High-speed phase, dielectric mirror

l 1550 nm

Algorithm: Weighted Gerchberg Saxton

Language: C++, CUDA

GPU: Quadro FX 5600

Bus: PCIe

Target : 5x5 grid, D = 32 pixels


What it looks like

What it looks like


3d printed living muscle stem cells

3D printed living muscle stem cells


Living neural networks prior art

Living neural networks – prior art


Live cell pick and place

Live cell pick-and-place

http://optics.fjfi.cvut.cz/?q=en/node/529


Fabrication process steps

Fabrication process steps

1. Create polymer micro-fluidic chip (electrodes and covers not shown)

2. Inject neurons in growth solution

3. Optically pick and place neurons in electrodes

4. Neuron processes extend down ECM-coated channels

5. Final network structure


Network layout

Network layout

Device Layout

Copper

Polymer

Neuron electrode


Neuron confinement and electrical interface

Neuron confinement and electrical interface

Electroplated

Copper

Polymer


Fabricated neuron electrode

Fabricated neuron electrode


Larger view with interconnect channels

Larger view with interconnect channels


Manual test of holographic trapping system for cell placement

Manual test of holographic trapping system for cell placement

Neuron

well

Electrode + constraint

Bead insertion in electrode

Neuron precursor cell in channel

Trap

Trap

Electrode + constraint

PC12 cell

Bead

Wall

Wall

Channel


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