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PART Ⅰ 色谱基础知识 南方医院临床试验研究中心 李 科 联系电话: 62787825/62787815 PowerPoint PPT Presentation


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PART Ⅰ 色谱基础知识 南方医院临床试验研究中心 李 科 联系电话: 62787825/62787815. 一、色谱起源 色谱法起源于 20 世纪初, 1906 年 俄国植物学家 米哈伊茨维特 用 碳酸钙 填充竖立的玻璃管,以 石油醚 洗脱植物色素的提取液,经过一段时间洗脱之后,植物色素在碳酸钙柱中实现分离,由一条色带分散为数条平行的色带。由于这一实验将混合的植物色素分离为不同的色带,因此茨维特将这种方法命名为色谱法。. 色谱. 石油醚. 组分. 色素. 碳酸钙颗粒. 色谱的主要目的是对混合物中的目标物分离和定量.

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PART Ⅰ 色谱基础知识 南方医院临床试验研究中心 李 科 联系电话: 62787825/62787815

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62787825/62787815


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201906


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  • ppm-ppb -

  • 1-100L


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  • Gaschromatography GC.

  • Liquid chromatography LC.


Hplc vs gc

HPLC VS GC

  • ,


Hplc vs gc1

HPLC VS GC

  • ,


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HPLC

  • (NP-HPLC)

  • (RP-HPLC)

  • (RPIC)

  • (IEC)

  • (SEC)

  • (Chiral separation mode)


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  • :


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HPLC

  • C18 (ODS) type

  • C8 (octyl) type

  • C4 (butyl) type

  • Phenyl type

  • TMS type

  • Cyano type

Non-polar property

-Si-C18H37

Si


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: /


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HPLC

-Si-CH2CH2CH2CN

-Si-CH2CH2CH2NH2

-Si-CH2CH2CH2OCH(OH)-CH2(OH)

Si

Si

Silica gel

Modified Si


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  • NH2APSCNCPS/


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  • 3-10m

  • 80-100A


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  • h

  • W W h

  • t0

  • tR,

  • (t'R)t'R= tR-t0

  • k k= t'R t0 k


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  • n n=5.54(tR/ W h)2

  • n=16(tR/W)2

  • H H= L/n L:


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  • R) R= 2tR2-tR1/W1+W2)

  • R<1,

  • R=1,98%

  • R=1.5,99.7%


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  • T T= W0.05/ 2d0.05

  • W0.05 W0.05 5% D0.05 5%

  • T0.951.05T<0.95T>1.05


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  • HPLC


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(nm)


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HPLC

  • 1

  • 2

  • 3

  • 4

  • 5

  • 6


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  • HPLC18.20.22um


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0.45um


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66

,70%


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1

2


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A

A

B

B

B


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MeOH / H2O = 6 / 4

MeOH / H2O = 8 / 2

( column : ODS type )


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95%

MeOH

30%


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/


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/

    • ()


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ODS

1150kgf/cm215cm

24050

3PH27.5


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LC

  • UV/VIS

  • PDA

  • RID

  • CDD

  • RF

  • L-ECD

  • MS

  • ELSD


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