Objective . The aim of the experiment is to determine : Total bilirubin Direct bilirubin Indirect bilirubin , Contents of human serum . . Introduction . The liver responsible for many important metabolic functions > 100 lab tests associated with liver function Major hepatic functions Protein synthesis Regulation of carbohydrate metabolism Regulation of lipoproteins Detoxification of drugs and chemicals Excretion of bile Formation of bilirubin .