新世代流式細胞儀. Partec GmbH from Münster Germany. 派特科技有限公司. Fluorescence Based Flow Cytometry. Invented in 1968 in the University of M ün ster, Germany at the Institute of Radiobiology by Professor Dr.Wolfgang Göhde.
from Münster Germany
The ICP 11.
The first commercial flow Cytometer is born -the legendary ICP-11, developed in Münster and distributed by
Phywe AG, Göttingen.
Electronics size and fashion changed since that time...
Award for outstanding product quality of theon the
最後一個客人進入時資料也得到了螢光顯微鏡 vs. 流式細胞儀
Laminar Coaxial Flow
The Bernoulli Effect
Viscous drag along walls. Particles move to low pressure area
High angle scatter : SSC
Low angle scatter : FSC
Fluorescence at longer wavelengths.
(自體螢光 auto fluorescence)
Cell Viability: Cell Dying by Apoptosis
Tumor Cell Analysis
Simultaneous DNA content and Cell-Surface Immuno- fluorescence Analysis
Matching for Solid Organ Transplantation
Cell Membrane Potential Analysis
Sperm Chromatin Structure Assay
Intracellular Ionized Ca
Lysosomal Proton Pump Activity
“No-Wash” procedures for Nuclei Antigen Detection
Simultaneous Analysis of Cellular RNA & DNA content
PRINS & FISH
Reticulocyte Analysis & Maturity Index
Various light sources:
Blue 488 nm 200 mW
Red 635 nm 25 mW
Green NdYAG 532 nm 50 mW
Many More to Come
…雷射發展之趨勢 – 固態雷射
Highest Stability and Highest Precision
Power Connection: Regular 100/240 VAC or Car Battery (12 V)
Single Platform Volumetric Absolute Counting
Up to 5 Parameters (FSC, SSC, FL1, FL2, FL3)
Single Laser SystemUV/Violet, Blue, Green, and Red Solid State Lasers
New Level of Price / Performance Ratio
Supported by the use of a PMT for the FSC parameter, the small white blood cell platelets (PLT) can safely be discriminated from red blood cells (RBC) in the scatter plot.
DAPI (DNA, FL4) and red staining (neutral lipids, FL3) of brewery yeasts after double excitation with HBO-lamp (UV) and argon ion laser (488 nm, 20 mW)
Measurement of RFP-expression (FL2: orange-red) of an E. coli population after excitation with an YAG-laser (523 nm, 50 mW): 40% of the E. coli population show the red fluorescence due to RFP-expression.
Bacteria in chocolate drink. left: control, right: contaminated sample.
The discrimination between “chocolate” particles and bacteria is based on spectral analysis in the two fluorescence channels.
The measurement volume is defined by mechanical measurement, rather than reference beads.
Partec流式細胞儀使用與火星計畫 Sujourner 相同的馬達
Routine concentration analysis on CD4/CD8 for HIV monitoring.
The concentration of subpopulations can as well be analyzed “offline”, after analysis.
Rare event concentration analysis on CD34+.