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(a)

(a). Fold RLUs. -. -. 1 g. 1 g. 1 g. 1 g. pCMV-p65wt-tag. -. 1 g. 0.5 g. 1 g. 2 g. -. pCMV- NH 2 p65 -tag. Fold p24. -. -. 1 g. 1 g. 1 g. 1 g. pCMV-p65wt-tag. -. 1 g. 0.5 g. 1 g. 2 g. -. pCMV- NH 2 p65 -tag. (b). pCMV-  NH 2 p65 -tag.

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(a)

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  1. (a) Fold RLUs - - 1g 1g 1g 1g pCMV-p65wt-tag - 1g 0.5g 1g 2g - pCMV-NH2p65-tag Fold p24 - - 1g 1g 1g 1g pCMV-p65wt-tag - 1g 0.5g 1g 2g - pCMV-NH2p65-tag (b) pCMV-NH2p65-tag pCMV-NH2p65-tag pCMV-p65wt-tag pCMV-p65wt-tag pCMV-tag1 pCMV-tag1 75 p65wt-tag IP: āFLAG IB: āp65 COOH NH2p65-tag 50 Cytosol Nucleus

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