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Soybean SE Transformation Induction Plant and zygotic embryo production ( 6-8 weeks )

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Soybean SE Transformation Induction Plant and zygotic embryo production ( 6-8 weeks ) Induction on D40 ( 6-8 weeks ) Proliferation on solid D20 ( 4 weeks ) Proliferation on FNL ( 4 weeks ). Soybean SE Transformation- cont. II. Gene Transfer, Selection, Proliferation & Maturation

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slide1

Soybean SE Transformation

  • Induction
  • Plant and zygotic embryo production (6-8 weeks)
  • Induction on D40 (6-8 weeks)
  • Proliferation on solid D20 (4 weeks)
  • Proliferation on FNL (4 weeks)
slide2

Soybean SE Transformation- cont.

  • II. Gene Transfer, Selection, Proliferation &Maturation
  • Place 20-25 SE clumps on D20
  • Shoot
  • Continue on D20 (1 week)
  • Transfer into FNL/H20 for seln. (7–10 weeks)
  • Isolate green, well formed globular SEs, grow on multi-well plate (5 wks)
  • Proliferate selected material in FNL (5 weeks)
  • Mature healthy green embryos matured on SHaM (4-5 weeks)
  • Desiccation (5-7 days) Select healthy matured
      • SEs for lipid and/or
      • other analysis
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Soybean SE Transformation- cont.

  • III. Conversion & Seed Production
  • Desiccated SEs
  • Germination (1 week)
  • Plant hardening (3 weeks)
  • Transfer hardy plantlets into pots, grow-out in greenhouse
  • Harvest mature seeds (10-16 weeks)
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